Detailed information NTG0020951

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BSU_02820
• Genome accession: NC_000964
• Coordinates: 304430..305551 (+)
• Length: 373 a.a.
• NCBI ID: NP_388164.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299430..310551

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_02780 (BSU02780)	ycdA	299438..300502 (-)	1065	NP_388160.1	lipoprotein involved in swarming behaviour	-
BSU_02785 (BSU02785)	-	300525..300656 (-)	132	YP_009513941.1	hypothetical protein	-
BSU_02790 (BSU02790)	ycdB	300830..302248 (+)	1419	NP_388161.1	hypothetical protein	-
BSU_02800 (BSU02800)	ycdC	302435..303796 (+)	1362	NP_388162.1	hypothetical protein	-
BSU_02810 (BSU02810)	cwlK	303804..304307 (-)	504	NP_388163.1	murein L,D:-endopeptidase	-
BSU_02820 (BSU02820)	rapF	304430..305551 (+)	1122	NP_388164.1	response regulator aspartate phosphatase	Regulator
BSU_02830 (BSU02830)	ycdF	305658..306434 (+)	777	NP_388165.1	putative oxidoreductase	-
BSU_02840 (BSU02840)	ycdG	306459..308144 (+)	1686	NP_388166.1	putative oligo-carbohydrate hydrolase	-
BSU_02850 (BSU02850)	znuA	308332..309291 (+)	960	NP_388167.1	Zn(II)-binding lipoprotein	-
BSU_02860 (BSU02860)	znuC	309347..310042 (+)	696	NP_388168.1	Zn(II) transporter (ATP-binding protein)	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=20951 BSU_02820 NP_388164.1 304430..305551(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=20951 BSU_02820 NP_388164.1 304430..305551(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405