Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CRI72_RS13265 Genome accession   NZ_CP023815
Coordinates   2634214..2634873 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain IMT16316     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2629214..2639873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRI72_RS13235 - 2629330..2630277 (+) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -
  CRI72_RS13240 - 2630274..2631161 (+) 888 WP_000614960.1 MurR/RpiR family transcriptional regulator -
  CRI72_RS13245 ygiN 2631206..2631520 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  CRI72_RS13250 mdaB 2631551..2632132 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CRI72_RS13255 ygiZ 2632490..2632822 (+) 333 WP_022646136.1 DUF2645 family protein -
  CRI72_RS13260 qseC 2632868..2634217 (-) 1350 WP_022646135.1 quorum sensing histidine kinase QseC -
  CRI72_RS13265 ciaR 2634214..2634873 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CRI72_RS13270 ygiW 2635025..2635417 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CRI72_RS13275 ygiV 2635470..2635952 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  CRI72_RS13280 ygiS 2636061..2637668 (+) 1608 WP_022646134.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=208743 CRI72_RS13265 WP_001221493.1 2634214..2634873(-) (ciaR) [Escherichia coli strain IMT16316]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=208743 CRI72_RS13265 WP_001221493.1 2634214..2634873(-) (ciaR) [Escherichia coli strain IMT16316]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365