Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CP957_RS03950 Genome accession   NZ_CP023673
Coordinates   732642..733301 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain SMN013SH2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 727642..738301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CP957_RS03925 (CP957_03890) yqiA 727710..728291 (+) 582 WP_000105733.1 esterase YqiA -
  CP957_RS03930 (CP957_03895) parE 728320..730212 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CP957_RS03935 (CP957_03900) ygiN 730260..730574 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CP957_RS03940 (CP957_03905) mdaB 730605..731186 (-) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  CP957_RS03945 (CP957_03910) qseC 731296..732645 (-) 1350 WP_000673376.1 quorum sensing histidine kinase QseC -
  CP957_RS03950 (CP957_03915) ciaR 732642..733301 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CP957_RS03955 (CP957_03920) ygiW 733453..733845 (+) 393 WP_001364958.1 OB fold stress tolerance protein YgiW -
  CP957_RS03960 (CP957_03925) ygiV 733898..734380 (+) 483 WP_000183486.1 GyrI-like domain-containing protein -
  CP957_RS03975 (CP957_03935) parC 734926..737184 (+) 2259 WP_062881426.1 DNA topoisomerase IV subunit A -
  CP957_RS03980 (CP957_03940) plsC 737417..738154 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=208327 CP957_RS03950 WP_001221502.1 732642..733301(-) (ciaR) [Escherichia coli strain SMN013SH2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=208327 CP957_RS03950 WP_001221502.1 732642..733301(-) (ciaR) [Escherichia coli strain SMN013SH2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACTGGCCTTAGTAAAATGGGTTTTAGTGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TTGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment