Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PFAS1_RS15570 Genome accession   NZ_CP018319
Coordinates   3358844..3360184 (-) Length   446 a.a.
NCBI ID   WP_076029704.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3353844..3365184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS15555 (PFAS1_15535) - 3354120..3357239 (+) 3120 WP_076029701.1 pilus assembly protein -
  PFAS1_RS15560 (PFAS1_15540) - 3357254..3357649 (+) 396 WP_076029702.1 type IV pilin protein -
  PFAS1_RS15565 (PFAS1_15545) thiO 3357706..3358806 (+) 1101 WP_076029703.1 glycine oxidase ThiO -
  PFAS1_RS15570 (PFAS1_15550) pilR 3358844..3360184 (-) 1341 WP_076029704.1 sigma-54-dependent transcriptional regulator Regulator
  PFAS1_RS15575 (PFAS1_15555) pilS 3360187..3361776 (-) 1590 WP_076029705.1 sensor histidine kinase Regulator
  PFAS1_RS15580 (PFAS1_15560) - 3361766..3361999 (-) 234 WP_074871005.1 PP0621 family protein -
  PFAS1_RS15585 (PFAS1_15565) - 3362122..3363144 (-) 1023 WP_076029706.1 outer membrane protein assembly factor BamD -
  PFAS1_RS15590 (PFAS1_15570) rluD 3363296..3364258 (+) 963 WP_007942311.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PFAS1_RS15595 (PFAS1_15575) pgeF 3364255..3364980 (+) 726 WP_076029707.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 49690.97 Da        Isoelectric Point: 6.5936

>NTDB_id=208036 PFAS1_RS15570 WP_076029704.1 3358844..3360184(-) (pilR) [Pseudomonas frederiksbergensis strain AS1]
MSPRQKVLIVDDEPDIRELLEITLGRMKLDTFSARNLSEAQALLSREAFDLCLTDMRLPDGTGLELVQHIQQRCPNVPVA
MITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVSSALRLPAPGGTGIAIDRRLLGDSPPMRALRKHIDKLARSQAPV
YISGESGSGKELVARLIHEQGPRASKPFVPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVADL
PLAMQVKLLRAIQEKAVRGVGEQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLNVIELRVPSLRERRDDIESLASH
VLKRLAISTGQPAAKLHPQALDALKSYRFPGNVRELENVLERAHTLCENKQIETSDLRLAEGNCNPEGGVPDLTQIDNLE
DYLENIERNLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKFGLD

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=208036 PFAS1_RS15570 WP_076029704.1 3358844..3360184(-) (pilR) [Pseudomonas frederiksbergensis strain AS1]
ATGAGCCCACGGCAAAAAGTCCTGATCGTCGATGACGAGCCGGATATCCGCGAACTCCTGGAAATTACCCTGGGACGGAT
GAAACTCGACACGTTCAGTGCCCGCAACCTCAGCGAAGCCCAGGCCCTGCTGTCCCGAGAGGCGTTCGACTTGTGCCTGA
CCGACATGCGCTTGCCGGACGGCACCGGTCTTGAGCTGGTGCAACACATCCAGCAACGCTGCCCAAACGTACCGGTGGCG
ATGATCACCGCTTATGGCAGCCTGGAAACGGCGATCAACGCCCTCAAGGCCGGTGCCTTTGACTTCCTGACCAAACCGGT
GGACCTCACTCGCCTGCGGGAACTGGTCAGCAGCGCGCTTCGGTTGCCTGCGCCGGGCGGCACCGGCATCGCCATCGATC
GCCGGCTGCTGGGTGATTCACCCCCCATGCGCGCCTTGCGCAAACATATCGACAAACTGGCCCGCAGCCAGGCGCCGGTG
TACATCAGCGGCGAATCCGGCAGCGGCAAGGAATTGGTCGCGCGACTGATCCATGAGCAAGGGCCGCGCGCCAGCAAACC
GTTTGTCCCGGTCAATTGCGGGGCGATTCCGTCGGAATTAATGGAAAGCGAATTTTTCGGGCATCGCAAAGGCAGCTTCA
GCGGTGCCATCGAAGACAAACCCGGACTGTTCCAGGCAGCCCATGGCGGCACCCTGTTCCTCGACGAAGTGGCTGATTTG
CCGCTGGCGATGCAGGTCAAGCTGCTACGGGCCATACAGGAAAAAGCCGTTCGCGGTGTCGGTGAGCAACAGGAAACCGT
GGTCGATGTGCGTATCCTGTGCGCCACCCACAAAGACCTCGACGCCGAAGTCGCCGCCGAACGTTTTCGCCAGGATTTGT
ACTACCGGTTGAACGTGATCGAACTACGAGTGCCGTCCTTGCGCGAACGCCGTGACGACATTGAATCCCTCGCCAGCCAT
GTGCTCAAACGCCTGGCCATCAGCACCGGCCAACCCGCCGCAAAACTCCACCCCCAAGCCCTAGACGCGCTGAAAAGCTA
TCGCTTCCCCGGCAATGTGCGGGAACTGGAGAACGTGCTCGAACGGGCACACACGTTGTGCGAAAACAAACAAATTGAAA
CCAGCGACCTGCGACTGGCCGAAGGCAACTGCAACCCGGAAGGCGGTGTGCCGGACCTGACGCAGATCGATAATCTGGAG
GACTACCTGGAAAACATCGAACGCAACCTTATCCTCCAGGCCCTGGAAGAAACCCGCTGGAACCGCACGGCGGCGGCTCA
GCGGTTGAATCTTTCATTCCGGTCGATGCGTTACAGGCTCAAAAAATTCGGGTTGGATTGA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)

Response regulator receiver domain (Response_reg)

(7-116)

Sigma-54 interaction domain (Sigma54_activat)

(137-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

79.007

99.327

0.785
  pilR Acinetobacter baumannii strain A118

49.455

100

0.509

Multiple sequence alignment