Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PFAS1_RS15575 Genome accession   NZ_CP018319
Coordinates   3360187..3361776 (-) Length   529 a.a.
NCBI ID   WP_076029705.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3355187..3366776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS15560 (PFAS1_15540) - 3357254..3357649 (+) 396 WP_076029702.1 type IV pilin protein -
  PFAS1_RS15565 (PFAS1_15545) thiO 3357706..3358806 (+) 1101 WP_076029703.1 glycine oxidase ThiO -
  PFAS1_RS15570 (PFAS1_15550) pilR 3358844..3360184 (-) 1341 WP_076029704.1 sigma-54-dependent transcriptional regulator Regulator
  PFAS1_RS15575 (PFAS1_15555) pilS 3360187..3361776 (-) 1590 WP_076029705.1 sensor histidine kinase Regulator
  PFAS1_RS15580 (PFAS1_15560) - 3361766..3361999 (-) 234 WP_074871005.1 PP0621 family protein -
  PFAS1_RS15585 (PFAS1_15565) - 3362122..3363144 (-) 1023 WP_076029706.1 outer membrane protein assembly factor BamD -
  PFAS1_RS15590 (PFAS1_15570) rluD 3363296..3364258 (+) 963 WP_007942311.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PFAS1_RS15595 (PFAS1_15575) pgeF 3364255..3364980 (+) 726 WP_076029707.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58580.46 Da        Isoelectric Point: 7.0405

>NTDB_id=208037 PFAS1_RS15575 WP_076029705.1 3360187..3361776(-) (pilS) [Pseudomonas frederiksbergensis strain AS1]
MIAETSSPGSKQAQRLLRLYHLYRLSIGITLVLLIASNMDNQLLTFSNDDLLRSGSWFYLVLNILLVVFLGNTRRPAQLF
SLALTDVLLLCGLFYAAGGVASAIGNLLIVSVAISNTLLRGRIGLLIAAVGALGIVGLSFLLSFSHPATPNDYLQVGTLG
ALCFAAALLVQGLIRRLEVSETLAEQRASEVVSLEALNALILQRMRTGILVLDDKRRVQLANHSALNLLGRESLAGQLID
DYLPSLVERLQLWLNNPTLRPQSLKVASSGLELQPSFIALDQSPHHQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLQESEELNSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTVAQRLDLKPWLEQFVNETRARTIEH
QQIHLRIGSGDFKTLMDPDQLTQILDNLLRNAWRHSALNHDQAQAWLELFVDPDSQLPVLEILDDGPGVAPEHQVHLFEP
FFTTSTQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=208037 PFAS1_RS15575 WP_076029705.1 3360187..3361776(-) (pilS) [Pseudomonas frederiksbergensis strain AS1]
GTGATCGCTGAGACCTCCAGTCCCGGCAGCAAACAGGCCCAGCGACTGCTGCGCCTCTATCACCTCTACCGTTTGAGCAT
CGGCATCACCCTGGTGCTGTTGATCGCCAGCAACATGGACAACCAGTTGCTGACGTTCAGCAATGACGACCTTCTGCGCA
GTGGCAGCTGGTTTTACTTGGTGCTGAACATCCTGCTGGTGGTATTCCTCGGGAACACCCGGCGCCCGGCGCAACTGTTC
AGCCTGGCGCTGACCGATGTCCTGCTGCTGTGCGGCTTGTTCTACGCCGCCGGCGGTGTGGCCAGCGCCATCGGCAACTT
GCTGATCGTATCGGTCGCCATCAGCAACACTTTGTTGCGAGGGCGCATCGGCCTGCTGATTGCCGCCGTTGGCGCGCTCG
GCATTGTCGGGCTGAGCTTCCTCCTCAGTTTCAGCCATCCCGCCACCCCCAACGATTACCTGCAAGTCGGTACGCTCGGC
GCCTTGTGTTTTGCTGCGGCATTGCTGGTGCAAGGGCTGATCCGACGCCTGGAGGTCAGCGAAACCCTCGCCGAACAACG
GGCCAGCGAAGTCGTCAGCCTTGAAGCCCTCAACGCCCTGATCCTGCAACGCATGCGTACCGGCATCCTGGTTCTCGACG
ACAAGCGGCGCGTTCAACTGGCCAACCACAGCGCCTTGAACCTGTTAGGCAGGGAAAGTCTCGCAGGCCAACTGATCGAC
GACTACTTGCCCAGCCTGGTCGAACGCCTGCAACTGTGGCTCAACAATCCAACGCTGCGTCCCCAGAGCCTGAAAGTCGC
CAGCAGTGGTCTGGAGCTGCAACCGAGCTTCATTGCCCTGGACCAAAGCCCGCACCACCAGACTCTGGTCTTTCTCGAAG
ACCTCGCGCAAATCGCTCAACAAGCCCAGCAGTTGAAGCTAGCCGCCCTTGGTCGCCTGACCGCCGGTATCGCCCATGAA
ATCCGCAATCCACTGGGCGCCGTTAGTCATGCCGCGCAGCTATTGCAGGAATCAGAGGAACTGAACAGCGCGGATCGACG
TCTGACCCAGATTATTCAAGACCACTCTCAGCGAATGAATCGGGTAATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AAACCGTAGCGCAGCGGCTTGATCTGAAGCCGTGGCTGGAACAGTTCGTCAACGAAACCCGCGCGAGGACGATTGAGCAT
CAGCAGATTCACCTGCGCATCGGCTCAGGAGACTTCAAAACCCTGATGGATCCAGACCAGTTGACTCAGATTCTCGACAA
TCTGTTGCGCAATGCCTGGCGCCATAGCGCCTTGAATCACGATCAAGCACAGGCTTGGCTCGAGCTGTTCGTCGACCCGG
ACAGCCAGCTGCCGGTCCTCGAGATCCTGGACGATGGCCCCGGCGTGGCACCGGAGCATCAGGTGCATTTGTTCGAACCG
TTCTTCACCACCAGCACCCAAGGCACCGGCCTGGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAGCCACGCCAAGGCGGCGGCTGCTTTCGCATCACTTTCGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.566

100

0.607

Multiple sequence alignment