Detailed information NTG0207393

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CO204_RS01205
• Genome accession: NZ_CP023477
• Coordinates: 231621..232055 (+)
• Length: 144 a.a.
• NCBI ID: WP_002263440.1
• Uniprot ID: Q8DS54
• Organism: Streptococcus mutans strain LAR01
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 226621..237055

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CO204_RS01175 (CO204_01175)	-	228243..228626 (+)	384	WP_002269807.1	DUF1033 family protein	-
CO204_RS01180 (CO204_01180)	comGA	228756..229697 (+)	942	WP_002264247.1	competence type IV pilus ATPase ComGA	Machinery gene
CO204_RS01185 (CO204_01185)	comGB	229633..230664 (+)	1032	WP_255262721.1	competence type IV pilus assembly protein ComGB	Machinery gene
CO204_RS01190 (CO204_01190)	comGC	230664..230978 (+)	315	WP_002263437.1	competence type IV pilus major pilin ComGC	Machinery gene
CO204_RS01195 (CO204_01195)	comGD	230965..231369 (+)	405	WP_002263438.1	competence type IV pilus minor pilin ComGD	Machinery gene
CO204_RS01200 (CO204_01200)	comGE	231341..231634 (+)	294	WP_002263439.1	competence type IV pilus minor pilin ComGE	Machinery gene
CO204_RS01205 (CO204_01205)	comGF	231621..232055 (+)	435	WP_002263440.1	competence type IV pilus minor pilin ComGF	Machinery gene
CO204_RS01210 (CO204_01210)	comGG	232033..232422 (+)	390	WP_002263441.1	competence type IV pilus minor pilin ComGG	Machinery gene
CO204_RS01215 (CO204_01215)	comGH	232477..233430 (+)	954	WP_002269808.1	class I SAM-dependent methyltransferase	Machinery gene
CO204_RS01220 (CO204_01220)	-	233489..234688 (+)	1200	WP_002263443.1	acetate kinase	-
CO204_RS01225 (CO204_01225)	tnpA	234901..235364 (+)	464	Protein_206	IS200/IS605 family transposase	-
CO204_RS01235 (CO204_01235)	-	235992..236237 (+)	246	WP_002263444.1	helix-turn-helix transcriptional regulator	-
CO204_RS01240 (CO204_01240)	-	236206..236646 (+)	441	WP_002264006.1	hypothetical protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16266.75 Da
• Isoelectric Point: 9.9512

>NTDB_id=207393 CO204_RS01205 WP_002263440.1 231621..232055(+) (comGF) [Streptococcus mutans strain LAR01]
MLKSKLKAFTLLECLLALLVIAGSVSVYNGLTKAISSNIHYLSKNQEDAWLLFCQQFRSELEGTTLQKLDSNKLYIQKNN
QSLAFGKSKASDFRKTNSDGRGYQPMLTEIKAANFSQSGKIIKLDLTFKDGLERTFVYAFHEKN

Nucleotide

• Length: 435 bp

>NTDB_id=207393 CO204_RS01205 WP_002263440.1 231621..232055(+) (comGF) [Streptococcus mutans strain LAR01]
TTGTTAAAAAGTAAATTGAAAGCTTTCACACTTTTAGAGTGTCTCCTTGCTTTGCTTGTCATTGCTGGTAGTGTATCTGT
TTATAATGGTCTAACTAAAGCTATTAGTAGTAATATTCATTACTTATCAAAAAATCAAGAAGATGCTTGGTTACTTTTTT
GTCAGCAATTTCGGTCTGAACTTGAAGGAACGACTTTGCAAAAATTGGATAGCAATAAGCTTTATATTCAAAAAAATAAT
CAAAGTTTAGCCTTTGGTAAATCAAAAGCAAGTGATTTTAGAAAGACTAACAGTGATGGACGTGGTTATCAGCCTATGTT
GACGGAGATCAAAGCTGCTAATTTTAGTCAATCAGGAAAAATTATTAAATTAGACCTTACTTTCAAAGATGGTTTAGAGA
GGACTTTTGTTTATGCTTTTCATGAAAAAAATTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (36-133)

3D structure

• AlphaFold DB: Q8DS54

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	100	100	1
comGF	Streptococcus mutans UA140	99.306	100	0.993
comGF	Lactococcus lactis subsp. cremoris KW2	43.262	97.917	0.424
comGF/cglF	Streptococcus pneumoniae Rx1	40.299	93.056	0.375
comGF/cglF	Streptococcus pneumoniae D39	40.299	93.056	0.375
comGF/cglF	Streptococcus pneumoniae R6	40.299	93.056	0.375
comGF/cglF	Streptococcus pneumoniae TIGR4	40.299	93.056	0.375
comGF/cglF	Streptococcus mitis NCTC 12261	38.806	93.056	0.361