Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BSR08_RS09430 Genome accession   NZ_CP018184
Coordinates   1718933..1719550 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain KH2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1713933..1724550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR08_RS09405 (BSR08_09550) rpsT 1714235..1714501 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  BSR08_RS09410 (BSR08_09555) holA 1714516..1715559 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  BSR08_RS22785 - 1715599..1715748 (-) 150 WP_003229985.1 hypothetical protein -
  BSR08_RS09415 (BSR08_09560) yqzM 1715789..1715923 (+) 135 WP_003229983.1 YqzM family protein -
  BSR08_RS09420 (BSR08_09565) comEC 1715963..1718293 (-) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BSR08_RS09425 (BSR08_09570) comEB 1718297..1718866 (-) 570 WP_003229978.1 ComE operon protein 2 -
  BSR08_RS09430 (BSR08_09575) comEA 1718933..1719550 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  BSR08_RS09435 (BSR08_09580) comER 1719634..1720455 (+) 822 WP_014480313.1 late competence protein ComER -
  BSR08_RS09440 (BSR08_09585) yqeM 1720521..1721264 (-) 744 WP_014480314.1 class I SAM-dependent methyltransferase -
  BSR08_RS09445 (BSR08_09590) rsfS 1721261..1721617 (-) 357 WP_014480315.1 ribosome silencing factor -
  BSR08_RS09450 (BSR08_09595) yqeK 1721635..1722195 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BSR08_RS09455 (BSR08_09600) nadD 1722185..1722754 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  BSR08_RS09460 (BSR08_09605) yhbY 1722766..1723056 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BSR08_RS09465 (BSR08_09610) aroE 1723050..1723892 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=206709 BSR08_RS09430 WP_014480312.1 1718933..1719550(-) (comEA) [Bacillus subtilis strain KH2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=206709 BSR08_RS09430 WP_014480312.1 1718933..1719550(-) (comEA) [Bacillus subtilis strain KH2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment