Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PA4528 Genome accession   NC_002516
Coordinates   5072695..5073567 (+) Length   290 a.a.
NCBI ID   NP_253218.1    Uniprot ID   P22610
Organism   Pseudomonas aeruginosa PAO1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5067695..5078567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA4524 nadC 5068032..5068880 (+) 849 NP_253214.1 nicotinate-nucleotide pyrophosphorylase -
  PA4525 pilA 5069082..5069531 (-) 450 NP_253215.1 type 4 fimbrial protein PilA Machinery gene
  PA4526 pilB 5069763..5071463 (+) 1701 NP_253216.1 type 4 fimbrial biogenesis protein PilB Machinery gene
  PA4528 pilD 5072695..5073567 (+) 873 NP_253218.1 type 4 prepilin peptidase PilD Machinery gene
  PA4529 coaE 5073564..5074175 (+) 612 NP_253219.1 dephospho-CoA kinase -
  PA4530 - 5074172..5074372 (+) 201 NP_253220.1 zinc-binding protein -
  PA4531 - 5074409..5074618 (-) 210 NP_253221.1 hypothetical protein -
  PA4532 - 5074724..5075413 (-) 690 NP_253222.1 hypothetical protein -
  PA4533 - 5075410..5075880 (-) 471 NP_253223.1 hypothetical protein -
  PA4534 - 5075877..5076302 (-) 426 NP_253224.1 hypothetical protein -
  PA4535 - 5076435..5077064 (+) 630 NP_253225.1 hypothetical protein -
  PA4536 - 5077061..5077510 (+) 450 NP_253226.1 hypothetical protein -
  PA4537 - 5077536..5077706 (+) 171 NP_253227.1 hypothetical protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31869.92 Da        Isoelectric Point: 7.7068

>NTDB_id=20667 PA4528 NP_253218.1 5072695..5073567(+) (pilD) [Pseudomonas aeruginosa PAO1]
MPLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=20667 PA4528 NP_253218.1 5072695..5073567(+) (pilD) [Pseudomonas aeruginosa PAO1]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22610

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

50.19

90.69

0.455

Multiple sequence alignment