Detailed information    

insolico Bioinformatically predicted

Overview


Name   sinI   Type   Regulator
Locus tag   BSF20_RS14630 Genome accession   NZ_CP018152
Coordinates   2798269..2798442 (-) Length   57 a.a.
NCBI ID   WP_003153105.1    Uniprot ID   A7Z6M7
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   inhibit the expression of sinR (predicted from homology)   
Competence regulation

Genomic Context


Location: 2793269..2803442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS14580 (BSF20_14285) comGD 2793390..2793827 (+) 438 WP_072588444.1 competence type IV pilus minor pilin ComGD Machinery gene
  BSF20_RS14585 (BSF20_14290) comGE 2793811..2794125 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  BSF20_RS14590 (BSF20_14295) comGF 2794139..2794534 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  BSF20_RS14595 (BSF20_14300) comGG 2794535..2794912 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  BSF20_RS14600 (BSF20_14305) - 2794969..2795148 (+) 180 WP_003153093.1 YqzE family protein -
  BSF20_RS14605 (BSF20_14310) - 2795188..2795517 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  BSF20_RS14610 (BSF20_14315) tapA 2795776..2796447 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  BSF20_RS14615 (BSF20_14320) sipW 2796419..2797003 (+) 585 WP_003153100.1 signal peptidase I SipW -
  BSF20_RS14620 (BSF20_14325) tasA 2797067..2797852 (+) 786 WP_003153102.1 biofilm matrix protein TasA -
  BSF20_RS14625 (BSF20_14330) sinR 2797900..2798235 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  BSF20_RS14630 (BSF20_14335) sinI 2798269..2798442 (-) 174 WP_003153105.1 anti-repressor SinI Regulator
  BSF20_RS14635 (BSF20_14340) - 2798619..2799413 (-) 795 WP_014305407.1 YqhG family protein -
  BSF20_RS14640 (BSF20_14345) - 2799431..2801101 (-) 1671 WP_003153107.1 DEAD/DEAH box helicase -
  BSF20_RS14645 (BSF20_14350) gcvT 2801524..2802624 (+) 1101 WP_058906182.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence


Protein


Download         Length: 57 a.a.        Molecular weight: 6695.72 Da        Isoelectric Point: 9.8170

>NTDB_id=206552 BSF20_RS14630 WP_003153105.1 2798269..2798442(-) (sinI) [Bacillus amyloliquefaciens strain LM2303]
MKNAKMEFSQLDKEWVELILEAQKANISLEEIRKYLLSHKKSSQHIQAARSHTVNPF

Nucleotide


Download         Length: 174 bp        

>NTDB_id=206552 BSF20_RS14630 WP_003153105.1 2798269..2798442(-) (sinI) [Bacillus amyloliquefaciens strain LM2303]
ATGAAAAATGCAAAAATGGAATTTTCGCAATTAGATAAGGAATGGGTTGAGCTGATATTAGAAGCCCAAAAAGCAAATAT
CAGCCTAGAAGAAATACGCAAATACCTGCTTTCGCATAAAAAGTCTTCTCAACATATCCAGGCAGCCAGAAGTCATACCG
TAAATCCTTTCTGA

Domains


Predicted by InterproScan.

(11-37)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A7Z6M7

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sinI Bacillus subtilis subsp. subtilis str. 168

70.175

100

0.702


Multiple sequence alignment