Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   BMJ42_RS12040 Genome accession   NZ_CP018136
Coordinates   2175927..2177225 (-) Length   432 a.a.
NCBI ID   WP_000867610.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain SP49     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2170927..2182225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMJ42_RS12020 (BMJ42_02282) rplI 2172082..2172534 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  BMJ42_RS12025 (BMJ42_02283) - 2172531..2174504 (-) 1974 WP_000715128.1 DHH family phosphoesterase -
  BMJ42_RS12030 (BMJ42_02284) hpf 2174640..2175188 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BMJ42_RS12035 (BMJ42_02285) comFC/cflB 2175268..2175930 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  BMJ42_RS12040 (BMJ42_02286) comFA/cflA 2175927..2177225 (-) 1299 WP_000867610.1 DEAD/DEAH box helicase Machinery gene
  BMJ42_RS12045 (BMJ42_02287) - 2177281..2177916 (+) 636 WP_000395739.1 YigZ family protein -
  BMJ42_RS12050 (BMJ42_02288) cysK 2178201..2179127 (+) 927 WP_000029874.1 cysteine synthase A -
  BMJ42_RS12055 (BMJ42_02289) tsf 2179248..2180288 (-) 1041 WP_000808063.1 translation elongation factor Ts -
  BMJ42_RS12060 (BMJ42_02290) rpsB 2180367..2181146 (-) 780 WP_000268466.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49577.51 Da        Isoelectric Point: 8.4382

>NTDB_id=206104 BMJ42_RS12040 WP_000867610.1 2175927..2177225(-) (comFA/cflA) [Streptococcus pneumoniae strain SP49]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=206104 BMJ42_RS12040 WP_000867610.1 2175927..2177225(-) (comFA/cflA) [Streptococcus pneumoniae strain SP49]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAGAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGTATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCGGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAATAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae D39

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae R6

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae TIGR4

98.843

100

0.988
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment