Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   B8B76_RS29905 Genome accession   NZ_CP023316
Coordinates   6284931..6285554 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PPF-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6279931..6290554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B8B76_RS29885 (B8B76_29835) aroB 6280526..6281632 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  B8B76_RS29890 (B8B76_29840) aroK 6281682..6282200 (-) 519 WP_003095833.1 shikimate kinase AroK -
  B8B76_RS29895 (B8B76_29845) pilQ 6282212..6284356 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  B8B76_RS29900 (B8B76_29850) pilP 6284410..6284934 (-) 525 WP_021263517.1 type 4a pilus biogenesis lipoprotein PilP -
  B8B76_RS29905 (B8B76_29855) pilO 6284931..6285554 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  B8B76_RS29910 (B8B76_29860) pilN 6285551..6286147 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  B8B76_RS29915 (B8B76_29865) pilM 6286147..6287211 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  B8B76_RS29920 (B8B76_29870) - 6287396..6289867 (+) 2472 WP_019484455.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=205926 B8B76_RS29905 WP_003103268.1 6284931..6285554(-) (pilO) [Pseudomonas aeruginosa strain PPF-1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=205926 B8B76_RS29905 WP_003103268.1 6284931..6285554(-) (pilO) [Pseudomonas aeruginosa strain PPF-1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTATCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401