Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CLC88_RS18665 Genome accession   NZ_CP023165
Coordinates   3668469..3669128 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O22:H8 strain RM10809-3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3663469..3674128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLC88_RS18645 (CLC88_19140) plsC 3663618..3664355 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  CLC88_RS18650 (CLC88_19145) parC 3664588..3666846 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CLC88_RS18655 (CLC88_19150) ygiV 3667392..3667874 (-) 483 WP_050864129.1 GyrI-like domain-containing protein -
  CLC88_RS18660 (CLC88_19155) ygiW 3667926..3668318 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CLC88_RS18665 (CLC88_19160) ciaR 3668469..3669128 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CLC88_RS18670 (CLC88_19165) qseC 3669125..3670473 (+) 1349 Protein_3573 quorum sensing histidine kinase QseC -
  CLC88_RS18675 (CLC88_19170) ygiZ 3670519..3670851 (-) 333 WP_000912120.1 DUF2645 family protein -
  CLC88_RS18680 (CLC88_19175) mdaB 3671170..3671769 (+) 600 WP_050864130.1 NADPH:quinone oxidoreductase MdaB -
  CLC88_RS18685 (CLC88_19180) ygiN 3671782..3672096 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CLC88_RS18690 (CLC88_19185) parE 3672144..3674036 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=205475 CLC88_RS18665 WP_001221502.1 3668469..3669128(+) (ciaR) [Escherichia coli O22:H8 strain RM10809-3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=205475 CLC88_RS18665 WP_001221502.1 3668469..3669128(+) (ciaR) [Escherichia coli O22:H8 strain RM10809-3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment