Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CGP99_RS08400 Genome accession   NZ_CP022906
Coordinates   1637639..1638136 (-) Length   165 a.a.
NCBI ID   WP_240316779.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 546     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632639..1643136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGP99_RS08365 (CGP99_01518) gcvPA 1632934..1634280 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CGP99_RS08370 (CGP99_01519) gcvT 1634300..1635391 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  CGP99_RS08375 (CGP99_01520) - 1635550..1636074 (-) 525 WP_001015117.1 shikimate kinase -
  CGP99_RS08380 - 1636064..1636210 (-) 147 WP_001789879.1 hypothetical protein -
  CGP99_RS08395 (CGP99_01521) - 1636483..1637655 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  CGP99_RS08400 (CGP99_01522) comGF 1637639..1638136 (-) 498 WP_240316779.1 competence type IV pilus minor pilin ComGF Machinery gene
  CGP99_RS08405 (CGP99_01523) comGE 1638054..1638353 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CGP99_RS08410 (CGP99_01524) comGD 1638340..1638786 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  CGP99_RS08415 (CGP99_01525) comGC 1638764..1639075 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  CGP99_RS08420 (CGP99_01526) comGB 1639089..1640159 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  CGP99_RS08425 (CGP99_01527) comGA 1640131..1641105 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  CGP99_RS08430 (CGP99_01528) - 1641157..1641780 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  CGP99_RS08435 (CGP99_01529) - 1641777..1642106 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CGP99_RS08440 (CGP99_01530) - 1642106..1643092 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19354.05 Da        Isoelectric Point: 10.0574

>NTDB_id=204345 CGP99_RS08400 WP_240316779.1 1637639..1638136(-) (comGF) [Staphylococcus aureus strain 546]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSVKSRLLCKMLYNVFTI
STYQN

Nucleotide


Download         Length: 498 bp        

>NTDB_id=204345 CGP99_RS08400 WP_240316779.1 1637639..1638136(-) (comGF) [Staphylococcus aureus strain 546]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCAGTCAAGTCCAGACTCCTGTGTAAAATGCTATACAATGTTTTTACCATT
TCTACTTATCAAAATTGA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(67-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

91.463

99.394

0.909
  comGF Staphylococcus aureus N315

95.862

87.879

0.842

Multiple sequence alignment