Detailed information NTG0202825

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BK055_RS13260
• Genome accession: NZ_CP017775
• Coordinates: 2710386..2711000 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain 9912D
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2705386..2716000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BK055_RS13235 (BK055_13235)	rpsT	2705689..2705955 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BK055_RS13240 (BK055_13240)	holA	2705972..2707013 (-)	1042	Protein_2617	DNA polymerase III subunit delta	-
BK055_RS21880	-	2707053..2707204 (-)	152	Protein_2618	hypothetical protein	-
BK055_RS13245 (BK055_13245)	-	2707245..2707379 (+)	135	WP_003152870.1	YqzM family protein	-
BK055_RS13250 (BK055_13250)	comEC	2707398..2709749 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BK055_RS13255 (BK055_13255)	-	2709750..2710319 (-)	570	WP_003152868.1	ComE operon protein 2	-
BK055_RS13260 (BK055_13260)	comEA	2710386..2711000 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
BK055_RS13265 (BK055_13265)	comER	2711059..2711880 (+)	822	WP_014305449.1	late competence protein ComER	-
BK055_RS13270 (BK055_13270)	-	2711949..2712686 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
BK055_RS13275 (BK055_13275)	rsfS	2712683..2713039 (-)	357	WP_003152864.1	ribosome silencing factor	-
BK055_RS13280 (BK055_13280)	yqeK	2713036..2713617 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BK055_RS13285 (BK055_13285)	-	2713607..2714176 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
BK055_RS13290 (BK055_13290)	yhbY	2714187..2714477 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BK055_RS13295 (BK055_13295)	aroE	2714471..2715313 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=202825 BK055_RS13260 WP_013352942.1 2710386..2711000(-) (comEA) [Bacillus velezensis strain 9912D]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=202825 BK055_RS13260 WP_013352942.1 2710386..2711000(-) (comEA) [Bacillus velezensis strain 9912D]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363