Detailed information NTG0202568

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BJN34_RS17510
• Genome accession: NZ_CP017757
• Coordinates: 3783815..3784717 (+)
• Length: 300 a.a.
• NCBI ID: WP_078197963.1
• Uniprot ID: -
• Organism: Cupriavidus necator strain NH9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3778815..3789717

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJN34_RS17490 (BJN34_17435)	-	3779777..3780088 (-)	312	WP_234824849.1	helix-turn-helix domain-containing protein	-
BJN34_RS17495 (BJN34_17440)	-	3780094..3780420 (-)	327	WP_078197960.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BJN34_RS17500 (BJN34_17445)	pilF	3780750..3782474 (+)	1725	WP_078197961.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BJN34_RS17505 (BJN34_17450)	-	3782540..3783811 (+)	1272	WP_078197962.1	type II secretion system F family protein	-
BJN34_RS17510 (BJN34_17455)	pilD	3783815..3784717 (+)	903	WP_078197963.1	A24 family peptidase	Machinery gene
BJN34_RS17515 (BJN34_17460)	coaE	3784791..3785417 (+)	627	WP_078197964.1	dephospho-CoA kinase	-
BJN34_RS17520 (BJN34_17465)	zapD	3785627..3786385 (+)	759	WP_078197965.1	cell division protein ZapD	-
BJN34_RS17525 (BJN34_17470)	-	3786403..3786591 (+)	189	WP_078197966.1	DNA gyrase inhibitor YacG	-
BJN34_RS17530 (BJN34_17475)	-	3786622..3788661 (-)	2040	WP_078197967.1	oligopeptide transporter, OPT family	-
BJN34_RS17535 (BJN34_17480)	-	3788755..3789201 (-)	447	WP_078197968.1	NUDIX domain-containing protein	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 32195.44 Da
• Isoelectric Point: 8.3375

>NTDB_id=202568 BJN34_RS17510 WP_078197963.1 3783815..3784717(+) (pilD) [Cupriavidus necator strain NH9]
MLSAWSASPYPTGSAPLLHALTALPPAFLVAIAALLGLVVGSFLNVVIHRMPRMMEREEANYIAELRGDPLPYPGRYNLM
VPRSACPHCGHAIAPWENVPVLSYLFLRGRCSACKAPISVRYPLVELACGVLSALVAWRFGPGGQALAALVLVWALLALT
MIDADTQLLPDQITLPLLWLGLLLNLGGLFVALPDAVIGAAAGYLALWTAYWLFRLVRGKEGMGFGDFKLMAALGAWFGW
QALPALVLLSSVAGVLFGLANIALRRQERDTPFPFGPFIALAGVVVMLFGPGVLPLFAWP

Nucleotide

• Length: 903 bp

>NTDB_id=202568 BJN34_RS17510 WP_078197963.1 3783815..3784717(+) (pilD) [Cupriavidus necator strain NH9]
ATGTTGTCTGCGTGGTCTGCTTCTCCCTACCCAACCGGCTCCGCGCCGCTGCTGCATGCGCTGACGGCGCTGCCACCCGC
CTTCCTGGTGGCAATCGCGGCGCTGCTCGGGCTGGTGGTCGGCAGCTTCCTCAATGTGGTCATCCACCGGATGCCGCGCA
TGATGGAGCGCGAAGAGGCCAACTATATCGCCGAGCTGCGCGGCGATCCGTTGCCCTACCCCGGCCGCTACAACCTGATG
GTGCCGCGCTCGGCCTGCCCGCATTGCGGTCACGCGATCGCGCCCTGGGAAAACGTGCCGGTGCTGAGCTACCTGTTCCT
GCGCGGACGCTGCTCGGCGTGCAAGGCGCCGATCAGCGTGCGCTATCCGCTGGTGGAGCTGGCCTGCGGCGTGCTGAGCG
CGCTGGTGGCGTGGCGCTTCGGCCCCGGCGGGCAGGCGCTGGCCGCGCTGGTGCTGGTGTGGGCGCTGCTGGCGCTGACC
ATGATCGATGCCGACACGCAATTGCTGCCGGACCAGATCACGCTGCCGCTGCTGTGGCTGGGCCTGCTGCTGAACCTGGG
CGGCCTGTTCGTGGCGCTGCCCGACGCGGTGATCGGCGCCGCTGCCGGTTACCTGGCGCTGTGGACGGCCTACTGGCTGT
TCCGGCTGGTGCGCGGCAAGGAAGGCATGGGCTTCGGCGACTTCAAGCTGATGGCGGCACTGGGGGCCTGGTTCGGCTGG
CAGGCGCTGCCGGCGCTGGTGCTGCTGTCGTCGGTGGCCGGCGTGCTGTTCGGGCTGGCCAATATCGCGCTGCGCCGCCA
GGAGCGCGATACGCCGTTCCCGTTCGGGCCCTTCATCGCGCTGGCCGGCGTGGTGGTGATGCTGTTCGGCCCCGGCGTGC
TGCCGCTGTTTGCCTGGCCATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (150-259)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (35-140)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.263	88.667	0.49
pilD	Vibrio campbellii strain DS40M4	50.909	91.667	0.467
pilD	Neisseria gonorrhoeae MS11	48.239	94.667	0.457
pilD	Acinetobacter baumannii D1279779	46.316	95	0.44
pilD	Acinetobacter nosocomialis M2	45.965	95	0.437