Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CIW80_RS09490 Genome accession   NZ_CP022686
Coordinates   1906582..1907241 (+) Length   219 a.a.
NCBI ID   WP_001221477.1    Uniprot ID   A0A0H2VCI3
Organism   Escherichia coli Nissle 1917     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1901582..1912241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CIW80_RS09475 (CIW80_09525) ygiS 1903787..1905394 (-) 1608 WP_001305104.1 ABC transporter substrate-binding protein -
  CIW80_RS09480 (CIW80_09530) ygiV 1905503..1905985 (-) 483 WP_000183488.1 GyrI-like domain-containing protein -
  CIW80_RS09485 (CIW80_09535) ygiW 1906038..1906430 (-) 393 WP_000712665.1 OB fold stress tolerance protein YgiW -
  CIW80_RS09490 (CIW80_09540) ciaR 1906582..1907241 (+) 660 WP_001221477.1 quorum sensing response regulator transcription factor QseB Regulator
  CIW80_RS09495 (CIW80_09545) qseC 1907238..1908587 (+) 1350 WP_000673390.1 quorum sensing histidine kinase QseC -
  CIW80_RS09500 (CIW80_09550) ygiZ 1908633..1908965 (-) 333 WP_000914696.1 DUF2645 family protein -
  CIW80_RS26425 - 1908972..1909682 (-) 711 WP_000834021.1 hypothetical protein -
  CIW80_RS26430 - 1909685..1910164 (-) 480 WP_000069056.1 Hcp family type VI secretion system effector -
  CIW80_RS09510 (CIW80_09560) mdaB 1910414..1910995 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CIW80_RS09515 (CIW80_09565) ygiN 1911026..1911340 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24756.74 Da        Isoelectric Point: 7.4218

>NTDB_id=202145 CIW80_RS09490 WP_001221477.1 1906582..1907241(+) (ciaR) [Escherichia coli Nissle 1917]
MRILLIEDDMLIGDGIKTGLSKMGFRVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=202145 CIW80_RS09490 WP_001221477.1 1906582..1907241(+) (ciaR) [Escherichia coli Nissle 1917]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTCGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TAGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VCI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment