Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BKN48_RS02250 Genome accession   NZ_CP017676
Coordinates   382471..383088 (-) Length   205 a.a.
NCBI ID   WP_021480063.1    Uniprot ID   -
Organism   Bacillus subtilis strain VV2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 377471..388088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKN48_RS02225 (BKN48_02225) rpsT 377772..378038 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  BKN48_RS02230 (BKN48_02230) holA 378053..379096 (-) 1044 WP_070547551.1 DNA polymerase III subunit delta -
  BKN48_RS21015 - 379136..379249 (-) 114 WP_122060479.1 hypothetical protein -
  BKN48_RS02235 (BKN48_02235) yqzM 379326..379460 (+) 135 WP_003229983.1 YqzM family protein -
  BKN48_RS02240 (BKN48_02240) comEC 379501..381831 (-) 2331 WP_070547552.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BKN48_RS02245 (BKN48_02245) comEB 381835..382404 (-) 570 WP_003229978.1 ComE operon protein 2 -
  BKN48_RS02250 (BKN48_02250) comEA 382471..383088 (-) 618 WP_021480063.1 competence protein ComEA Machinery gene
  BKN48_RS02255 (BKN48_02255) comER 383172..383993 (+) 822 WP_021480064.1 late competence protein ComER -
  BKN48_RS02260 (BKN48_02260) yqeM 384059..384802 (-) 744 WP_070547553.1 class I SAM-dependent DNA methyltransferase -
  BKN48_RS02265 (BKN48_02265) rsfS 384799..385155 (-) 357 WP_014480315.1 ribosome silencing factor -
  BKN48_RS02270 (BKN48_02270) yqeK 385173..385733 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BKN48_RS02275 (BKN48_02275) nadD 385723..386292 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  BKN48_RS02280 (BKN48_02280) yhbY 386304..386594 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BKN48_RS02285 (BKN48_02285) aroE 386588..387430 (-) 843 WP_070547554.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21928.73 Da        Isoelectric Point: 4.8417

>NTDB_id=201471 BKN48_RS02250 WP_021480063.1 382471..383088(-) (comEA) [Bacillus subtilis strain VV2]
MNWLNQHKKAIILAAFTAVFTAIMIFLATGKNKEPVKQAVPTETENTVIKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=201471 BKN48_RS02250 WP_021480063.1 382471..383088(-) (comEA) [Bacillus subtilis strain VV2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTTTACGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGATAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGAATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

36.406

100

0.385
  comEA Staphylococcus aureus N315

35.023

100

0.371

Multiple sequence alignment