Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CGU46_RS11885 Genome accession   NZ_CP022525
Coordinates   2490784..2491407 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain Ocean-1175     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2485784..2496407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGU46_RS11870 (CGU46_11870) - 2486471..2488942 (-) 2472 WP_019484455.1 penicillin-binding protein 1A -
  CGU46_RS11875 (CGU46_11875) pilM 2489127..2490191 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CGU46_RS11880 (CGU46_11880) pilN 2490191..2490787 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CGU46_RS11885 (CGU46_11885) pilO 2490784..2491407 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  CGU46_RS11890 (CGU46_11890) pilP 2491404..2491928 (+) 525 WP_021263517.1 type 4a pilus biogenesis lipoprotein PilP -
  CGU46_RS11895 (CGU46_11895) pilQ 2491982..2494126 (+) 2145 WP_094069650.1 type 4a pilus secretin PilQ Machinery gene
  CGU46_RS11900 (CGU46_11900) aroK 2494138..2494656 (+) 519 WP_003095833.1 shikimate kinase AroK -
  CGU46_RS11905 (CGU46_11905) aroB 2494706..2495812 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=201211 CGU46_RS11885 WP_003103268.1 2490784..2491407(+) (pilO) [Pseudomonas aeruginosa strain Ocean-1175]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=201211 CGU46_RS11885 WP_003103268.1 2490784..2491407(+) (pilO) [Pseudomonas aeruginosa strain Ocean-1175]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTATCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401