Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   BJK08_RS03485 Genome accession   NZ_CP017602
Coordinates   720710..721159 (-) Length   149 a.a.
NCBI ID   WP_027228447.1    Uniprot ID   A0A2S6F4D1
Organism   Legionella pneumophila strain D5945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 715710..726159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJK08_RS03465 (BJK08_03380) - 715982..716374 (-) 393 WP_027228443.1 hypothetical protein -
  BJK08_RS03470 (BJK08_03385) - 716511..716888 (+) 378 WP_027228444.1 hypothetical protein -
  BJK08_RS03475 (BJK08_03390) - 717088..718377 (+) 1290 WP_027228445.1 tetratricopeptide repeat protein -
  BJK08_RS03480 (BJK08_03395) comEC 718425..720632 (-) 2208 WP_027228446.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BJK08_RS03485 (BJK08_03400) pilE 720710..721159 (-) 450 WP_027228447.1 type IV pilin protein Machinery gene
  BJK08_RS03490 (BJK08_03405) - 721171..724680 (-) 3510 WP_027228448.1 pilus assembly protein -
  BJK08_RS03495 (BJK08_03410) - 724693..725205 (-) 513 WP_027228449.1 pilus assembly PilX family protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16426.72 Da        Isoelectric Point: 8.4634

>NTDB_id=200665 BJK08_RS03485 WP_027228447.1 720710..721159(-) (pilE) [Legionella pneumophila strain D5945]
MLNNRHFMKNKRMRQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide


Download         Length: 450 bp        

>NTDB_id=200665 BJK08_RS03485 WP_027228447.1 720710..721159(-) (pilE) [Legionella pneumophila strain D5945]
ATGCTCAATAATCGACATTTTATGAAGAATAAGCGCATGAGACAATCTGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAGTCCCGTCGTGCCGATGCCC
ATGCTACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCTGCGGCGTGTGGCGCC
TTACCGGCATTTCCGCAAACAACACCAAATGGGTACTATACGATCAATATTTCAAACCTTACAGCTACAACGTATACCTT
AACGGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATCAATCAAGCGAATGTAAAAACAG
CAGTAGATTCCTCTGCTAATGCCCAGCCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-38)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(39-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6F4D1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila str. Paris

96.644

100

0.966
  pilE Legionella pneumophila strain ERS1305867

96.644

100

0.966

Multiple sequence alignment