Detailed information NTG0200626

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BJK09_RS05070
• Genome accession: NZ_CP017601
• Coordinates: 1102426..1103490 (+)
• Length: 354 a.a.
• NCBI ID: WP_011213353.1
• Uniprot ID: A0A3A6VL78
• Organism: Legionella pneumophila strain D6026
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1097426..1108490

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJK09_RS05060 (BJK09_04965)	-	1098468..1100852 (-)	2385	WP_011213351.1	penicillin-binding protein 1A	-
BJK09_RS05065 (BJK09_04970)	-	1101002..1102009 (-)	1008	WP_011213352.1	hypothetical protein	-
BJK09_RS05070 (BJK09_04975)	pilM	1102426..1103490 (+)	1065	WP_011213353.1	type IV pilus assembly protein PilM	Machinery gene
BJK09_RS05075 (BJK09_04980)	pilN	1103494..1104042 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
BJK09_RS05080 (BJK09_04985)	pilO	1104056..1104655 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
BJK09_RS05085 (BJK09_04990)	pilP	1104652..1105236 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
BJK09_RS05090 (BJK09_04995)	pilQ	1105240..1107336 (+)	2097	WP_011213357.1	type IV pilus secretin PilQ	Machinery gene
BJK09_RS05095 (BJK09_05000)	aroK	1107782..1108309 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38788.79 Da
• Isoelectric Point: 5.0367

>NTDB_id=200626 BJK09_RS05070 WP_011213353.1 1102426..1103490(+) (pilM) [Legionella pneumophila strain D6026]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKRLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=200626 BJK09_RS05070 WP_011213353.1 1102426..1103490(+) (pilM) [Legionella pneumophila strain D6026]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAGATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A3A6VL78

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.655	100	0.427
pilM	Acinetobacter baumannii D1279779	42.373	100	0.424
comM	Acinetobacter baylyi ADP1	40.96	100	0.41