Detailed information    

experimental Experimentally validated

Overview

Name   ruvA   Type   Machinery gene
Locus tag   C694_RS04485 Genome accession   NC_018939
Coordinates   932832..933383 (-) Length   183 a.a.
NCBI ID   WP_000635172.1    Uniprot ID   O25549
Organism   Helicobacter pylori 26695     
Function   homologous recombination   
Homologous recombination

Function

Inactivation of recA, recB, recN, ruvA, ruvB, and ruvC significantly reduced the number of recombinant clones.


Genomic Context

Location: 927832..938383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS04460 (C694_04490) ruvC 929801..930274 (-) 474 WP_001221153.1 crossover junction endodeoxyribonuclease RuvC Machinery gene
  C694_RS04470 (C694_04500) - 930405..931137 (+) 733 Protein_846 NYN domain-containing protein -
  C694_RS04475 (C694_04505) - 931617..932039 (-) 423 WP_000857970.1 hypothetical protein -
  C694_RS09045 - 932471..932740 (-) 270 Protein_848 hypothetical protein -
  C694_RS04485 (C694_04525) ruvA 932832..933383 (-) 552 WP_000635172.1 Holliday junction branch migration protein RuvA Machinery gene
  C694_RS04490 (C694_04530) - 933409..935253 (-) 1845 WP_000051272.1 FapA family protein -
  C694_RS04495 (C694_04535) murJ 935346..936806 (+) 1461 WP_000913560.1 murein biosynthesis integral membrane protein MurJ -
  C694_RS04500 (C694_04540) cysS 936807..938204 (+) 1398 WP_000471359.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20175.80 Da        Isoelectric Point: 8.4748

>NTDB_id=1488 C694_RS04485 WP_000635172.1 932832..933383(-) (ruvA) [Helicobacter pylori 26695]
MIVGLIGVVEKISALEVHIEVQGVVYGVQVSMRTAALLEAGQKARLKILQVIKEDAHLLYGFLEEGEKILFERLLKINGV
GGRIALAILSSFSPNEFESIIATKEVKRLQQVPGIGKKLADKIMVDLIGFFIQDENRPARNEVFLALESLGFKSAEINQV
LKTLKPNLSIEAAIKEALQQLRS

Nucleotide


Download         Length: 552 bp        

>NTDB_id=1488 C694_RS04485 WP_000635172.1 932832..933383(-) (ruvA) [Helicobacter pylori 26695]
ATGATAGTGGGTTTGATAGGGGTTGTGGAAAAAATCTCCGCTTTAGAAGTGCATATAGAAGTGCAAGGGGTTGTTTATGG
GGTGCAAGTTTCTATGCGAACGGCTGCTTTGCTTGAAGCGGGCCAAAAAGCGCGTTTGAAAATCTTGCAAGTGATCAAAG
AAGATGCACATCTTTTATACGGGTTTTTAGAAGAGGGCGAAAAAATCCTTTTTGAAAGGCTTTTAAAAATCAATGGGGTA
GGGGGGCGTATCGCTTTAGCCATTCTTTCAAGCTTTTCGCCGAACGAATTTGAAAGCATTATCGCCACCAAAGAAGTCAA
AAGACTCCAGCAAGTCCCAGGTATAGGGAAAAAGCTCGCTGATAAGATCATGGTGGATTTGATTGGCTTTTTCATTCAAG
ATGAAAACAGACCCGCACGCAATGAGGTTTTTTTAGCCCTAGAGAGTTTGGGCTTTAAAAGCGCTGAAATCAACCAAGTC
TTAAAAACCCTAAAACCCAATCTCAGCATAGAAGCAGCGATTAAAGAAGCCCTACAACAACTGCGCTCTTAA

Domains

Predicted by InterProScan.

RuvA, C-terminal domain (RuvA_C)

(139-181)

RuvA N terminal domain (RuvA_N)

(1-61)

Helix-hairpin-helix domain (HHH_5)

(71-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O25549

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Stefan Kulick et al. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS One 3(11):e3797. [PMID: 19030104]