Detailed information    

experimental Experimentally validated

Overview

Name   ruvC   Type   Machinery gene
Locus tag   C694_RS04460 Genome accession   NC_018939
Coordinates   929801..930274 (-) Length   157 a.a.
NCBI ID   WP_001221153.1    Uniprot ID   Q1CSZ5
Organism   Helicobacter pylori 26695     
Function   homologous recombination   
Homologous recombination

Function

Inactivation of recA, recB, recN, ruvA, ruvB, and ruvC significantly reduced the number of recombinant clones.


Genomic Context

Location: 924801..935274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS04450 (C694_04480) - 925585..927102 (-) 1518 WP_000247370.1 catalase -
  C694_RS04455 (C694_04485) - 927425..929800 (+) 2376 WP_000479960.1 TonB-dependent receptor -
  C694_RS04460 (C694_04490) ruvC 929801..930274 (-) 474 WP_001221153.1 crossover junction endodeoxyribonuclease RuvC Machinery gene
  C694_RS04470 (C694_04500) - 930405..931137 (+) 733 Protein_846 NYN domain-containing protein -
  C694_RS04475 (C694_04505) - 931617..932039 (-) 423 WP_000857970.1 hypothetical protein -
  C694_RS09045 - 932471..932740 (-) 270 Protein_848 hypothetical protein -
  C694_RS04485 (C694_04525) ruvA 932832..933383 (-) 552 WP_000635172.1 Holliday junction branch migration protein RuvA Machinery gene
  C694_RS04490 (C694_04530) - 933409..935253 (-) 1845 WP_000051272.1 FapA family protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17424.43 Da        Isoelectric Point: 9.4379

>NTDB_id=1490 C694_RS04460 WP_001221153.1 929801..930274(-) (ruvC) [Helicobacter pylori 26695]
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVNEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1490 C694_RS04460 WP_001221153.1 929801..930274(-) (ruvC) [Helicobacter pylori 26695]
ATGCGTATTTTAGGAATAGATCCGGGCAGTAGGAAATGCGGGTATGCTATCATTTCTCACGCTTCTAACAAGCTTTCTTT
AATCACGGCCGGGTTCATTAATATCACCACGACACGCTTGCAAGAACAGATTTTAGATTTGATAGAAGCCTTAGATTGCT
TATTGGATCGTTACGAAGTCAATGAAGTGGCGATTGAAGATATTTTCTTTGGGTATAACCCAAAAAGCGTGATCAAGCTC
GCGCAATTCAGGGGAGCGTTGTCCTTAAAGATTTTAGAAAGGATTGGTAATTTTAGCGAATACACGCCCTTACAAGTCAA
AAAAGCCCTGACCGGTAACGGGAAAGCCGCTAAAGAACAAGTAGCTTTTATGGTCAAGCGCTTGCTTAACATCACAAGCG
AAATCAAGCCTTTGGATATTAGCGATGCGATAGCCGTTGCTATCACGCATGCGCAACGCTTAAAGCTCCATTAA

Domains

Predicted by InterProScan.

Crossover junction endodeoxyribonuclease RuvC (RuvC)

(3-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1CSZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Florent Ailloud et al. (2022) The Helicobacter pylori UvrC Nuclease Is Essential for Chromosomal Microimports after Natural Transformation. MBio 13(4):e0181122. [PMID: 35876509]
[2] Stefan Kulick et al. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS One 3(11):e3797. [PMID: 19030104]