Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CGC46_RS24290 Genome accession   NZ_CP022407
Coordinates   4561536..4562195 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O121:H19 strain 16-9255     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4556536..4567195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGC46_RS24265 (CGC46_24305) ygiN 4557437..4557751 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CGC46_RS24270 (CGC46_24310) mdaB 4557782..4558363 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CGC46_RS30715 - 4558613..4559092 (+) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  CGC46_RS30720 - 4559095..4559805 (+) 711 WP_000834029.1 hypothetical protein -
  CGC46_RS24280 (CGC46_24320) ygiZ 4559812..4560144 (+) 333 WP_000914691.1 DUF2645 family protein -
  CGC46_RS24285 (CGC46_24325) qseC 4560190..4561539 (-) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  CGC46_RS24290 (CGC46_24330) ciaR 4561536..4562195 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CGC46_RS24295 (CGC46_24335) ygiW 4562347..4562739 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CGC46_RS24300 (CGC46_24340) ygiV 4562792..4563274 (+) 483 WP_000183502.1 GyrI-like domain-containing protein -
  CGC46_RS24310 (CGC46_24350) parC 4563819..4566077 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CGC46_RS24315 (CGC46_24355) plsC 4566310..4567047 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=200598 CGC46_RS24290 WP_001221502.1 4561536..4562195(-) (ciaR) [Escherichia coli O121:H19 strain 16-9255]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=200598 CGC46_RS24290 WP_001221502.1 4561536..4562195(-) (ciaR) [Escherichia coli O121:H19 strain 16-9255]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365