Detailed information NTG0199538

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: CES94_RS04015
• Genome accession: NZ_CP022154
• Coordinates: 764213..764872 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain ABWA45
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 759213..769872

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CES94_RS03985 (CES94_03975)	ygiN	760114..760428 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
CES94_RS03990 (CES94_03980)	mdaB	760459..761040 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
CES94_RS29880 (CES94_03985)	-	761290..761769 (+)	480	WP_000065333.1	Hcp family type VI secretion system effector	-
CES94_RS29885 (CES94_03990)	-	761772..762482 (+)	711	WP_000834029.1	hypothetical protein	-
CES94_RS04005 (CES94_03995)	ygiZ	762489..762821 (+)	333	WP_000914691.1	DUF2645 family protein	-
CES94_RS04010 (CES94_04000)	qseC	762867..764216 (-)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
CES94_RS04015 (CES94_04005)	ciaR	764213..764872 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
CES94_RS04020 (CES94_04010)	ygiW	765024..765416 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
CES94_RS04025 (CES94_04015)	ygiV	765469..765951 (+)	483	WP_000609258.1	GyrI-like domain-containing protein	-
CES94_RS04030 (CES94_04020)	mqsR	766156..766452 (+)	297	WP_000415583.1	type II toxin-antitoxin system toxin MqsR	-
CES94_RS04035 (CES94_04025)	mqsA	766454..766849 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
CES94_RS04040 (CES94_04030)	ygiS	766982..768589 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=199538 CES94_RS04015 WP_001221493.1 764213..764872(-) (ciaR) [Escherichia coli strain ABWA45]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=199538 CES94_RS04015 WP_001221493.1 764213..764872(-) (ciaR) [Escherichia coli strain ABWA45]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365