Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BG605_RS05300 Genome accession   NZ_CP017505
Coordinates   1029547..1030743 (+) Length   398 a.a.
NCBI ID   WP_006248403.1    Uniprot ID   A0A249A0I8
Organism   Mannheimia haemolytica strain 32619     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024547..1035743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG605_RS05265 (BG605_05195) proB 1024943..1026027 (-) 1085 Protein_1036 glutamate 5-kinase -
  BG605_RS05275 (BG605_05205) folA 1026139..1026633 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG605_RS05280 (BG605_05210) - 1026753..1026983 (+) 231 WP_006248398.1 YgjV family protein -
  BG605_RS05285 (BG605_05215) radA 1027038..1028417 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  BG605_RS05290 (BG605_05220) pilA 1028598..1029077 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  BG605_RS05295 (BG605_05225) - 1029098..1029541 (+) 444 Protein_1041 pilus assembly protein PilB -
  BG605_RS05300 (BG605_05230) pilC 1029547..1030743 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG605_RS05305 (BG605_05235) - 1030746..1031402 (+) 657 WP_006248404.1 prepilin peptidase -
  BG605_RS05310 (BG605_05240) coaE 1031438..1032073 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  BG605_RS05315 (BG605_05245) yacG 1032055..1032240 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  BG605_RS05320 (BG605_05250) holA 1032241..1033266 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  BG605_RS05325 (BG605_05255) lptE 1033309..1033797 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45186.75 Da        Isoelectric Point: 9.4698

>NTDB_id=199224 BG605_RS05300 WP_006248403.1 1029547..1030743(+) (pilC) [Mannheimia haemolytica strain 32619]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=199224 BG605_RS05300 WP_006248403.1 1029547..1030743(+) (pilC) [Mannheimia haemolytica strain 32619]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCTATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415

Multiple sequence alignment