Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BG561_RS04615 Genome accession   NZ_CP017502
Coordinates   918376..918855 (+) Length   159 a.a.
NCBI ID   WP_020831302.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 32852     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913376..923855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG561_RS04585 (BG561_04500) - 913949..914713 (-) 765 WP_006248395.1 TSUP family transporter -
  BG561_RS04590 (BG561_04505) proB 914713..915804 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  BG561_RS04595 (BG561_04510) folA 915916..916410 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG561_RS04600 (BG561_04515) - 916530..916760 (+) 231 WP_006251345.1 YgjV family protein -
  BG561_RS04605 (BG561_04520) radA 916815..918194 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  BG561_RS04615 (BG561_04525) pilA 918376..918855 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  BG561_RS04620 (BG561_04530) pilB 918876..920279 (+) 1404 WP_006251341.1 GspE/PulE family protein Machinery gene
  BG561_RS04625 (BG561_04535) pilC 920263..921459 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG561_RS04630 (BG561_04540) - 921462..922118 (+) 657 WP_006248404.1 prepilin peptidase -
  BG561_RS04635 (BG561_04545) coaE 922154..922789 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  BG561_RS04640 (BG561_04550) yacG 922771..922956 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16855.17 Da        Isoelectric Point: 8.7964

>NTDB_id=199155 BG561_RS04615 WP_020831302.1 918376..918855(+) (pilA) [Mannheimia haemolytica strain 32852]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=199155 BG561_RS04615 WP_020831302.1 918376..918855(+) (pilA) [Mannheimia haemolytica strain 32852]
ATGCAAAAAATTGTACATTTTCGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTAATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

57.143

88.05

0.503
  pilA Haemophilus influenzae Rd KW20

55.634

89.308

0.497

Multiple sequence alignment