Detailed information NTG0198396

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: BIZ51_RS03465
• Genome accession: NZ_CP017458
• Coordinates: 720709..721158 (-)
• Length: 149 a.a.
• NCBI ID: WP_027228447.1
• Uniprot ID: A0A2S6F4D1
• Organism: Legionella pneumophila subsp. fraseri strain Dallas 1E
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 715709..726158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BIZ51_RS03445 (BIZ51_03375)	-	715981..716373 (-)	393	WP_027228443.1	hypothetical protein	-
BIZ51_RS03450 (BIZ51_03380)	-	716510..716887 (+)	378	WP_027228444.1	hypothetical protein	-
BIZ51_RS03455 (BIZ51_03385)	-	717087..718376 (+)	1290	WP_027228445.1	tetratricopeptide repeat protein	-
BIZ51_RS03460 (BIZ51_03390)	comEC	718424..720631 (-)	2208	WP_027228446.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BIZ51_RS03465 (BIZ51_03395)	pilE	720709..721158 (-)	450	WP_027228447.1	type IV pilin protein	Machinery gene
BIZ51_RS03470 (BIZ51_03400)	-	721170..724679 (-)	3510	WP_027228448.1	pilus assembly protein	-
BIZ51_RS03475 (BIZ51_03405)	-	724692..725204 (-)	513	WP_027228449.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16426.72 Da
• Isoelectric Point: 8.4634

>NTDB_id=198396 BIZ51_RS03465 WP_027228447.1 720709..721158(-) (pilE) [Legionella pneumophila subsp. fraseri strain Dallas 1E]
MLNNRHFMKNKRMRQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=198396 BIZ51_RS03465 WP_027228447.1 720709..721158(-) (pilE) [Legionella pneumophila subsp. fraseri strain Dallas 1E]
ATGCTCAATAATCGACATTTTATGAAGAATAAGCGCATGAGACAATCTGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAGTCCCGTCGTGCCGATGCCC
ATGCTACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCTGCGGCGTGTGGCGCC
TTACCGGCATTTCCGCAAACAACACCAAATGGGTACTATACGATCAATATTTCAAACCTTACAGCTACAACGTATACCTT
AACGGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATCAATCAAGCGAATGTAAAAACAG
CAGTAGATTCCTCTGCTAATGCCCAGCCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: A0A2S6F4D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	96.644	100	0.966
pilE	Legionella pneumophila strain ERS1305867	96.644	100	0.966