Detailed information NTG0198087

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B195_RS03695
• Genome accession: NZ_CP017432
• Coordinates: 760180..761028 (-)
• Length: 282 a.a.
• NCBI ID: WP_003443838.1
• Uniprot ID: -
• Organism: Pseudomonas sp. Lz4W
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 755180..766028

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B195_RS03660 (B195_003605)	-	755709..757010 (-)	1302	WP_003443854.1	NAD(P)/FAD-dependent oxidoreductase	-
B195_RS03665 (B195_003610)	-	757074..757247 (-)	174	WP_003443851.1	DUF3094 family protein	-
B195_RS03670 (B195_003615)	-	757303..757830 (-)	528	WP_003443848.1	MOSC domain-containing protein	-
B195_RS03675 (B195_003620)	-	757827..758456 (-)	630	WP_003443844.1	DUF1780 domain-containing protein	-
B195_RS03680 (B195_003625)	-	758582..759268 (+)	687	WP_003443841.1	energy-coupling factor ABC transporter permease	-
B195_RS03685 (B195_003630)	yacG	759343..759546 (-)	204	WP_003443840.1	DNA gyrase inhibitor YacG	-
B195_RS03690 (B195_003635)	coaE	759543..760166 (-)	624	WP_003443839.1	dephospho-CoA kinase	-
B195_RS03695 (B195_003640)	pilD	760180..761028 (-)	849	WP_003443838.1	A24 family peptidase	Machinery gene
B195_RS03700 (B195_003645)	pilC	761031..762248 (-)	1218	WP_003443837.1	type II secretion system F family protein	Machinery gene
B195_RS03705 (B195_003650)	-	762470..762871 (+)	402	WP_003443834.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
B195_RS03710 (B195_003655)	-	763114..764610 (+)	1497	WP_003443832.1	DNA-3-methyladenine glycosylase 2	-
B195_RS03715 (B195_003660)	-	764607..765104 (+)	498	WP_003443830.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31267.44 Da
• Isoelectric Point: 8.3808

>NTDB_id=198087 B195_RS03695 WP_003443838.1 760180..761028(-) (pilD) [Pseudomonas sp. Lz4W]
MIEFFTRHPDAFIVCALILGLLVGSFINVLAWRLPRMLEQDWRAQARELLELPAAEKVPVYNLMLPRSHCPQCQHPLRLW
EKIPLLSYLLLGGKCSRCRTVISPRYPLTELACGLLSAFIAWHFGLSLQAGWMLVLTWGLLAICLIDAEHQIVPDVLVLP
LLWMGLLLNSFDTFTSLSQAVWGAALGYASLWSIFWIFKLATGKDGMGYGDFKLLAMLGAWGGIAVLPLTIVLSSLMGAI
AGLSLLALRRAKTSTPIPFAPYLAIAGWIGLLWGGQITAFYW

Nucleotide

• Length: 849 bp

>NTDB_id=198087 B195_RS03695 WP_003443838.1 760180..761028(-) (pilD) [Pseudomonas sp. Lz4W]
ATGATTGAATTTTTCACCCGCCACCCCGACGCTTTTATCGTCTGCGCCCTGATCCTCGGCTTGTTGGTGGGCAGCTTTAT
CAATGTCCTGGCCTGGCGCTTGCCCAGGATGCTCGAGCAGGACTGGCGAGCACAGGCCAGGGAATTGCTGGAGTTGCCCG
CAGCCGAAAAAGTGCCTGTGTATAACTTGATGCTGCCCCGCTCCCACTGCCCGCAATGCCAGCACCCGTTGCGCCTGTGG
GAAAAGATTCCGCTGCTCAGCTACCTGCTACTGGGTGGCAAGTGCTCCCGGTGCAGGACCGTTATCAGCCCGCGCTACCC
CCTGACCGAACTGGCTTGCGGATTATTGTCGGCGTTTATAGCCTGGCATTTCGGCCTGAGCCTGCAGGCGGGCTGGATGC
TGGTGCTGACCTGGGGGCTGCTGGCTATTTGCCTGATCGATGCCGAGCACCAGATCGTGCCCGATGTTCTGGTGCTGCCC
CTGCTCTGGATGGGCTTGCTGCTCAACAGCTTCGATACTTTTACCAGCCTGTCACAAGCGGTATGGGGCGCAGCGCTGGG
CTATGCAAGCCTGTGGTCGATCTTCTGGATCTTCAAGCTGGCCACCGGCAAGGACGGCATGGGCTATGGTGATTTCAAGC
TGCTGGCGATGCTCGGAGCCTGGGGCGGCATTGCAGTATTACCCTTGACCATTGTGCTCTCGTCACTGATGGGTGCGATC
GCAGGCCTGAGCCTGCTGGCATTACGCCGGGCCAAAACCTCCACCCCGATTCCCTTTGCACCCTATCTGGCAATTGCCGG
CTGGATTGGATTGCTCTGGGGTGGTCAAATAACCGCCTTCTATTGGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.41	100	0.486
pilD	Vibrio campbellii strain DS40M4	45.936	100	0.461
pilD	Acinetobacter nosocomialis M2	45.583	100	0.457
pilD	Acinetobacter baumannii D1279779	45.23	100	0.454
pilD	Neisseria gonorrhoeae MS11	47.451	90.426	0.429