Detailed information NTG0195487

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: A2U18_RS08210
• Genome accession: NZ_CP017091
• Coordinates: 1637592..1638089 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain ISU926 isolate ST398
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1632592..1643089

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A2U18_RS08185 (A2U18_07925)	gcvPB	1632754..1634226 (-)	1473	WP_071938166.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
A2U18_RS08190 (A2U18_07930)	gcvPA	1634219..1635565 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
A2U18_RS08195 (A2U18_07935)	gcvT	1635585..1636676 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
A2U18_RS08200 (A2U18_07940)	-	1636835..1637359 (-)	525	WP_001015120.1	shikimate kinase	-
A2U18_RS08205 (A2U18_07945)	-	1637349..1637495 (-)	147	WP_001789879.1	hypothetical protein	-
A2U18_RS08210 (A2U18_07950)	comGF	1637592..1638089 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
A2U18_RS08215 (A2U18_07955)	comGE	1638007..1638306 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
A2U18_RS08220 (A2U18_07960)	comGD	1638293..1638739 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
A2U18_RS08225 (A2U18_07965)	comGC	1638717..1639027 (-)	311	Protein_1548	competence type IV pilus major pilin ComGC	-
A2U18_RS08230 (A2U18_07970)	comGB	1639041..1640111 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
A2U18_RS08235 (A2U18_07975)	comGA	1640083..1641057 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
A2U18_RS08240 (A2U18_07980)	-	1641109..1641732 (-)	624	WP_031919558.1	MBL fold metallo-hydrolase	-
A2U18_RS08245 (A2U18_07985)	-	1641729..1642058 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
A2U18_RS08250 (A2U18_07990)	-	1642058..1643044 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=195487 A2U18_RS08210 WP_001825340.1 1637592..1638089(-) (comGF) [Staphylococcus aureus strain ISU926 isolate ST398]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=195487 A2U18_RS08210 WP_001825340.1 1637592..1638089(-) (comGF) [Staphylococcus aureus strain ISU926 isolate ST398]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97