Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABE28_RS15130 Genome accession   NZ_CP017080
Coordinates   3116716..3117780 (-) Length   354 a.a.
NCBI ID   WP_373921284.1    Uniprot ID   -
Organism   Peribacillus muralis strain G25-68     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3111716..3122780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABE28_RS15090 (ABE28_015090) - 3112446..3113243 (+) 798 WP_064463330.1 YqhG family protein -
  ABE28_RS15095 (ABE28_015095) - 3113472..3113657 (-) 186 WP_064463332.1 YqzE family protein -
  ABE28_RS15100 (ABE28_015100) comGG 3113740..3114126 (-) 387 WP_064463334.1 competence type IV pilus minor pilin ComGG -
  ABE28_RS15105 (ABE28_015105) comGF 3114119..3114598 (-) 480 WP_064463336.1 competence type IV pilus minor pilin ComGF -
  ABE28_RS15110 (ABE28_015110) - 3114546..3114884 (-) 339 WP_064463338.1 hypothetical protein -
  ABE28_RS15115 (ABE28_015115) comGD 3114871..3115281 (-) 411 WP_064463340.1 competence type IV pilus minor pilin ComGD -
  ABE28_RS15120 (ABE28_015120) comGC 3115322..3115630 (-) 309 WP_064463701.1 competence type IV pilus major pilin ComGC -
  ABE28_RS15125 (ABE28_015125) comGB 3115698..3116729 (-) 1032 WP_064463341.1 competence type IV pilus assembly protein ComGB -
  ABE28_RS15130 (ABE28_015130) comGA 3116716..3117780 (-) 1065 WP_373921284.1 competence type IV pilus ATPase ComGA Machinery gene
  ABE28_RS15135 (ABE28_015135) - 3117876..3118256 (-) 381 WP_064463343.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ABE28_RS15140 (ABE28_015140) - 3118470..3119171 (+) 702 WP_064463345.1 metalloregulator ArsR/SmtB family transcription factor -
  ABE28_RS15145 (ABE28_015145) - 3119255..3119497 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  ABE28_RS15150 (ABE28_015150) - 3119557..3120657 (-) 1101 WP_064463347.1 class I SAM-dependent methyltransferase -
  ABE28_RS15155 (ABE28_015155) - 3120858..3121484 (-) 627 WP_064463349.1 MBL fold metallo-hydrolase -
  ABE28_RS15160 (ABE28_015160) - 3121714..3121887 (+) 174 WP_064505148.1 DUF2759 domain-containing protein -
  ABE28_RS15165 (ABE28_015165) - 3122250..3122720 (-) 471 WP_064463351.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39496.23 Da        Isoelectric Point: 8.4623

>NTDB_id=195281 ABE28_RS15130 WP_373921284.1 3116716..3117780(-) (comGA) [Peribacillus muralis strain G25-68]
MNDLISIEKTAEKILTRAIQLSASDIHIFFRKEGPFIQFRIDNKLVPQETLSFFEAERLIAHLKFLAAMDIGEKRRPQSG
AITMNLADQVVGLRLSTLPTAYLESLVIRLIPQQKILPLEQLSLFPKTAQKLIALLKHSHGMLILTGPTGSGKTTTLYSL
LHHAHVMLTRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIIVGEIRDAETAKIAVRAALTGHLILT
TMHTRDAQGAISRLLEFGVSLLEIEQSLIGVTAQRLVELRCLACMGDCNCAKRASVYEMLYGKSLVDLIHMLKDEKGSAF
ISYRRLRDEIGKAVAMGYVDSQEYDRLVYQDGKK

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=195281 ABE28_RS15130 WP_373921284.1 3116716..3117780(-) (comGA) [Peribacillus muralis strain G25-68]
ATGAATGATTTGATATCAATTGAAAAGACAGCAGAAAAAATCCTTACTCGTGCCATTCAGTTATCGGCGTCGGACATCCA
CATTTTTTTTCGCAAGGAGGGACCATTTATCCAGTTCAGGATAGATAATAAGCTCGTACCTCAGGAAACGTTATCGTTCT
TTGAAGCTGAAAGGCTGATAGCCCATTTGAAGTTCCTTGCCGCGATGGATATTGGCGAGAAAAGGAGGCCGCAAAGTGGT
GCCATCACCATGAATTTAGCGGACCAAGTGGTAGGGCTCCGCCTCTCCACACTTCCCACTGCCTATCTCGAAAGTCTGGT
CATCCGCTTGATACCCCAACAGAAGATCCTTCCTCTGGAACAATTATCCTTATTCCCGAAAACCGCTCAAAAATTAATTG
CGCTTTTGAAGCACTCCCATGGCATGCTTATTCTTACAGGTCCAACAGGCAGCGGGAAAACGACGACTTTATATTCCCTG
CTCCATCATGCACATGTGATGCTTACTCGAAACATCATTACACTTGAAGATCCGATTGAAAATGTGTCCGAAAAGGTGCT
GCAGGTCCAAATTAATGAAAAGGCGGGCATTACCTATTCCGTCGGCTTAAAAGCTGTGCTGCGGCATGATCCTGATGTGA
TCATAGTGGGGGAAATCAGGGATGCAGAAACGGCAAAAATCGCGGTGCGTGCCGCATTGACCGGCCACTTGATTCTTACG
ACGATGCATACGAGGGATGCTCAAGGCGCCATCTCCAGATTGCTGGAATTTGGTGTAAGCTTGCTTGAGATTGAACAAAG
CTTGATTGGCGTGACGGCGCAGCGGTTGGTTGAACTTCGCTGCCTTGCATGCATGGGCGACTGTAATTGTGCGAAAAGGG
CAAGTGTGTATGAAATGCTATATGGAAAAAGTTTGGTCGACCTTATTCACATGCTTAAGGACGAAAAGGGCAGCGCGTTC
ATCAGCTATCGCCGATTAAGGGATGAAATTGGAAAAGCCGTTGCGATGGGCTATGTCGATTCACAGGAATATGACCGGTT
GGTATATCAAGATGGCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.338

100

0.565
  pilB Glaesserella parasuis strain SC1401

40.708

95.763

0.39
  pilB Haemophilus influenzae 86-028NP

38.15

97.74

0.373
  pilB Haemophilus influenzae Rd KW20

37.861

97.74

0.37

Multiple sequence alignment