Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   S101267_RS08820 Genome accession   NZ_CP021505
Coordinates   1653473..1655416 (+) Length   647 a.a.
NCBI ID   WP_013352220.1    Uniprot ID   A0A9P1NHU6
Organism   Bacillus amyloliquefaciens strain SRCM101267     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1648473..1660416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101267_RS08795 (S101267_01762) def 1648847..1649329 (+) 483 WP_013352215.1 peptide deformylase -
  S101267_RS08800 (S101267_01763) fmt 1649334..1650287 (+) 954 WP_013352216.1 methionyl-tRNA formyltransferase -
  S101267_RS08805 (S101267_01764) rsmB 1650274..1651617 (+) 1344 WP_065118558.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  S101267_RS08810 (S101267_01765) rlmN 1651621..1652712 (+) 1092 WP_013352218.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  S101267_RS08815 (S101267_01766) - 1652718..1653479 (+) 762 WP_013352219.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  S101267_RS08820 (S101267_01767) stkP 1653473..1655416 (+) 1944 WP_013352220.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  S101267_RS08825 (S101267_01768) rsgA 1655431..1656321 (+) 891 WP_013352221.1 ribosome small subunit-dependent GTPase A -
  S101267_RS08830 (S101267_01769) rpe 1656323..1656976 (+) 654 WP_013352222.1 ribulose-phosphate 3-epimerase -
  S101267_RS08835 (S101267_01770) - 1657044..1657688 (+) 645 WP_013352223.1 thiamine diphosphokinase -
  S101267_RS08840 spoVM 1657775..1657855 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  S101267_RS08845 (S101267_01771) rpmB 1657932..1658120 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  S101267_RS08850 (S101267_01772) - 1658380..1658742 (+) 363 WP_013352224.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71540.31 Da        Isoelectric Point: 5.1147

>NTDB_id=193502 S101267_RS08820 WP_013352220.1 1653473..1655416(+) (stkP) [Bacillus amyloliquefaciens strain SRCM101267]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYITAHGPLHPKEALSVMKQIVSAIAHAHHNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKMPFDGESAVSIALKHLQAETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAIAALQAETPAETDDKEQPSA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKEVSVPDVRGMKYEKAEALLEKNGLQADPDMTDIEDEKIEEGLMV
KTDPKAGSAVKEGSSVKLYKSIGKPKTQLIDVKGRPIDDAKKTLKDKGFKHVNVKEENDDSAAGTVIDQNPSAGTDMVVS
DDKVSLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKEAHSDDVPEGQVMKQEPAAGTAVKPGSDVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=193502 S101267_RS08820 WP_013352220.1 1653473..1655416(+) (stkP) [Bacillus amyloliquefaciens strain SRCM101267]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCGAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTTGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGACATTTATTATATTGTCATGGAATATGTGGAAGGCATGACGTTAAAGGAATACATAACCGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCTGTTATGAAGCAGATCGTTTCAGCCATCGCCCATGCCCATCATAACCATATCGTGCACCGGG
ATATCAAACCGCACAATATTTTGATAGATCATTTAGGCCATATCAAGGTGACGGATTTCGGCATCGCCACAGCCCTCACT
TCGACGACAATTACACATACGAATTCTGTGCTCGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCGGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGCAAAATGCCTTTTGACGGAGAAT
CAGCCGTCAGCATTGCGTTAAAGCATCTGCAAGCAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTGGAAAACATTATTTTAAAGGCAACCGCCAAAGATCCGTTTCACCGCTATGAAAGCGCAGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTCTCCGTGCAGGATGATGAGGAAATGACGAAAGCGATTC
CCGTTATTACTGACGGTGCGATAGCCGCGCTTCAGGCGGAAACGCCGGCAGAGACGGATGATAAAGAACAGCCGTCCGCC
AAAAAGAAGAAACGGAAATGGCCGTGGGTTTTGCTGGCTGTCTGCTTTATTTTCATAATGGCGGCTGTTCTTGCAGTCAC
CGTGTTCCCGTCTTTGTTTATGCCCAAAGAGGTAAGCGTCCCCGATGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGCCTTCAAGCGGATCCTGATATGACGGACATTGAAGATGAAAAAATCGAAGAGGGATTAATGGTC
AAAACTGATCCGAAAGCGGGTTCTGCCGTTAAAGAAGGCTCTTCTGTAAAGCTGTACAAAAGCATCGGGAAACCGAAAAC
CCAGCTGATTGACGTGAAAGGCCGGCCGATCGATGATGCGAAAAAAACGCTTAAGGATAAAGGCTTCAAACATGTCAATG
TAAAAGAAGAGAACGATGACAGTGCGGCTGGCACCGTAATTGATCAGAACCCATCAGCCGGAACCGACATGGTCGTAAGC
GATGATAAAGTCAGCTTAACAGTCAGCCTCGGACCGGCGGACGTTACGCTGAGAGACTTAAAAACATACAGTAAAGAAGC
GGCTTCCGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAGGAAGCTCACTCAGATGACGTGCCTGAGGGACAGG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTGATGTTGAAGTAACGTTTTCGCTCGGGCCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATCCCCTACGAACCCGAACATGAAGGGGACGAGCTCGAAGTGCA
AATCGCCATCGATGATAAAGACCACAGCATCTCTGATACGTATGACTCGTTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1NHU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366