Detailed information NTG0188771

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: BSR25_RS08510
• Genome accession: NZ_CP020604
• Coordinates: 1680911..1681357 (-)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1675911..1686357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSR25_RS08475 (BSR25_1676)	-	1676028..1676405 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
BSR25_RS08480 (BSR25_1677)	-	1676610..1677476 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
BSR25_RS08485 (BSR25_1678)	-	1677515..1678324 (-)	810	WP_010906310.1	metal ABC transporter permease	-
BSR25_RS08490 (BSR25_1679)	-	1678317..1679054 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
BSR25_RS08495 (BSR25_1680)	-	1679231..1680073 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
BSR25_RS08500 (BSR25_1681)	-	1680070..1680507 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
BSR25_RS08505 (BSR25_1682)	comGG	1680588..1680872 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
BSR25_RS08510 (BSR25_1683)	comGF	1680911..1681357 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
BSR25_RS08515	comGE	1681320..1681616 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
BSR25_RS08520 (BSR25_1685)	comGD	1681588..1682004 (-)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
BSR25_RS08525 (BSR25_1686)	comGC	1681979..1682248 (-)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
BSR25_RS08530 (BSR25_1687)	-	1682395..1682919 (-)	525	WP_014570795.1	GNAT family N-acetyltransferase	-
BSR25_RS08535 (BSR25_1688)	-	1682998..1683777 (-)	780	WP_058147788.1	peptidoglycan amidohydrolase family protein	-
BSR25_RS08540 (BSR25_1689)	-	1683777..1684076 (-)	300	WP_031559226.1	phage holin	-
BSR25_RS08545 (BSR25_1690)	-	1684089..1684439 (-)	351	WP_023349296.1	hypothetical protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=188771 BSR25_RS08510 WP_031296844.1 1680911..1681357(-) (comGF) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=188771 BSR25_RS08510 WP_031296844.1 1680911..1681357(-) (comGF) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comGF	Streptococcus mutans UA140	47.518	95.27	0.453
comGF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385