Detailed information    

insolico Bioinformatically predicted

Overview

Name   cclA/cilC   Type   Machinery gene
Locus tag   BSR25_RS08065 Genome accession   NZ_CP020604
Coordinates   1601008..1601673 (+) Length   221 a.a.
NCBI ID   WP_004254535.1    Uniprot ID   Q9CE22
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03     
Function   processing and translocation of ComGC; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1596008..1606673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR25_RS08035 (BSR25_1588) - 1597150..1597476 (-) 327 WP_010906269.1 lactococcin 972 family bacteriocin -
  BSR25_RS08040 (BSR25_1590) - 1597776..1598504 (-) 729 WP_004254546.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -
  BSR25_RS08045 (BSR25_1591) - 1598697..1598918 (+) 222 WP_010906270.1 YqgQ family protein -
  BSR25_RS08050 (BSR25_1592) - 1598952..1599923 (+) 972 WP_004254542.1 ROK family glucokinase -
  BSR25_RS08055 (BSR25_1593) - 1599926..1600312 (+) 387 WP_010906271.1 rhodanese-like domain-containing protein -
  BSR25_RS08060 (BSR25_1594) - 1600395..1600841 (-) 447 WP_004254538.1 Dps family protein -
  BSR25_RS08065 (BSR25_1595) cclA/cilC 1601008..1601673 (+) 666 WP_004254535.1 prepilin peptidase Machinery gene
  BSR25_RS08070 (BSR25_1596) dinB 1601624..1602715 (-) 1092 WP_010906272.1 DNA polymerase IV -
  BSR25_RS08075 (BSR25_1597) - 1602794..1603285 (-) 492 WP_010906273.1 prolyl-tRNA synthetase associated domain-containing protein -
  BSR25_RS08080 (BSR25_1598) - 1603364..1604857 (-) 1494 WP_010906274.1 basic amino acid/polyamine antiporter -
  BSR25_RS08085 (BSR25_1599) - 1605049..1606179 (-) 1131 WP_010906275.1 aminotransferase -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 25664.87 Da        Isoelectric Point: 8.8481

>NTDB_id=188765 BSR25_RS08065 WP_004254535.1 1601008..1601673(+) (cclA/cilC) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
MDILFIFLIGSIFGSFFGLIVDRIPLRKSIIFGRSHCDSCQQLLAYRDLIPIFSQISSGSHCRYCKSKIPYLYVFLEFSS
ALIFVLAWVNYLKPSQFLLIILSLILSAFDYRQHSFPFVIWFIFALSFILIFPLSPLFYFWLILAVLAEKFNLRVGSGDF
LWFFTASFSLTFLNEMLLLQIACSLGIIYYLITKKRVEIAFIPFLTIAYLMLLFGHQILSP

Nucleotide


Download         Length: 666 bp        

>NTDB_id=188765 BSR25_RS08065 WP_004254535.1 1601008..1601673(+) (cclA/cilC) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
ATGGACATTTTATTTATATTTTTAATTGGAAGTATTTTTGGCTCCTTTTTTGGCTTAATAGTTGACCGTATTCCACTAAG
AAAATCAATTATTTTTGGACGCTCACATTGTGATTCTTGTCAGCAACTTTTAGCCTATCGTGATCTAATTCCTATTTTTT
CTCAAATAAGCTCTGGCTCACATTGCCGTTATTGTAAAAGTAAAATTCCATATTTATATGTTTTTTTGGAGTTCTCATCA
GCTTTAATTTTTGTTTTAGCTTGGGTAAATTATCTTAAACCTAGTCAATTTTTACTGATTATTCTTTCACTAATTTTATC
TGCTTTTGATTATCGTCAGCACAGTTTTCCTTTTGTTATCTGGTTTATTTTTGCACTCAGTTTTATTTTAATATTTCCTC
TTTCTCCCCTTTTCTATTTTTGGCTTATTTTAGCCGTCTTAGCTGAAAAATTCAATTTAAGAGTTGGCAGCGGTGATTTT
TTATGGTTTTTTACCGCTTCATTTTCTCTAACTTTCTTAAACGAAATGCTGCTCCTTCAAATCGCTTGTTCACTTGGTAT
CATTTATTATCTAATCACAAAAAAACGAGTTGAAATTGCCTTCATCCCGTTTCTAACTATTGCATATCTAATGTTGCTTT
TTGGGCACCAAATTCTGTCACCGTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CE22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cclA/cilC Streptococcus mitis SK321

37.9

99.095

0.376
  cclA/cilC Streptococcus pneumoniae Rx1

38.14

97.285

0.371
  cclA/cilC Streptococcus pneumoniae D39

38.14

97.285

0.371
  cclA/cilC Streptococcus pneumoniae R6

38.14

97.285

0.371
  cclA/cilC Streptococcus pneumoniae TIGR4

37.674

97.285

0.367