Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BSY15_RS05500 Genome accession   NZ_CP016447
Coordinates   1229197..1230807 (-) Length   536 a.a.
NCBI ID   WP_069103953.1    Uniprot ID   A0A1B3PRB9
Organism   Acidovorax sp. RAC01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224197..1235807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY15_RS05480 (BSY15_1111) - 1225780..1226067 (+) 288 WP_069103949.1 hypothetical protein -
  BSY15_RS21860 (BSY15_1112) - 1226212..1227855 (-) 1644 WP_069103950.1 methyl-accepting chemotaxis protein -
  BSY15_RS05490 (BSY15_1113) - 1227975..1228442 (-) 468 WP_069103951.1 cache domain-containing protein -
  BSY15_RS05495 (BSY15_1114) - 1228719..1229171 (+) 453 WP_069103952.1 thioesterase family protein -
  BSY15_RS05500 (BSY15_1115) rpoN/rpoN1 1229197..1230807 (-) 1611 WP_069103953.1 RNA polymerase factor sigma-54 Machinery gene
  BSY15_RS05505 (BSY15_1116) lptB 1230907..1231674 (-) 768 WP_069106410.1 LPS export ABC transporter ATP-binding protein -
  BSY15_RS05510 (BSY15_1117) lptA 1231686..1232351 (-) 666 WP_069103954.1 lipopolysaccharide transport periplasmic protein LptA -
  BSY15_RS05515 (BSY15_1118) - 1232472..1233128 (-) 657 WP_069103955.1 thiol:disulfide interchange protein DsbA/DsbL -
  BSY15_RS05520 (BSY15_1119) - 1233198..1233845 (-) 648 WP_069103956.1 SPOR domain-containing protein -
  BSY15_RS05525 (BSY15_1120) argS 1233861..1235570 (-) 1710 WP_069103957.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 536 a.a.        Molecular weight: 58772.03 Da        Isoelectric Point: 4.6550

>NTDB_id=188315 BSY15_RS05500 WP_069103953.1 1229197..1230807(-) (rpoN/rpoN1) [Acidovorax sp. RAC01]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAPREEFGLDAADAPPQADDYSADDAIF
SGASSTTATSPEGQNESETPSGAGDEPDWSGDGTVEMAPNDAEWGGDAPPRTRNDSEGDEADATELARSHESLTAFLHRQ
ALGLRLSEVDCAALRFLIESLNDDGYLEESLEELAITLAGPDDLEQIEELVHRFTVAQRLLQSLEPVGVGARGLAECLTL
QLQAMAADEDGPADAATVQTALRICQQPLEMLAKRDVRRLTVLCSEGGASGEERTRAAMALIARLEPRPGRRFADVERNI
IIPDVIVRKAGREGAQRGRDTQQNFIVQLNPDVMPRLRVHDIYAGALRGHKGGEGHQGMQQRLQEARWFIKNIQQRFDTI
LRVSRAIVERQKSFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAV
RALIKQFVAAENAAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKTL

Nucleotide


Download         Length: 1611 bp        

>NTDB_id=188315 BSY15_RS05500 WP_069103953.1 1229197..1230807(-) (rpoN/rpoN1) [Acidovorax sp. RAC01]
ATGAAGCCCGGCCTGTCGCTGCGCGTATCGCAGCACCTTGCGCTCACGCCGCAGCTGCAGCAGTCCATCCGGCTGCTGCA
ACTGTCCACGCTGGAGCTATCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCGTTCCTGGAGCGCACGGCCGAGGAAG
CCCCGCGCGAGGAATTCGGCCTCGATGCCGCGGACGCACCGCCGCAGGCGGACGACTACAGTGCGGATGACGCTATATTT
TCAGGAGCATCAAGCACTACTGCAACAAGCCCTGAAGGCCAAAATGAGTCCGAAACACCCTCGGGCGCGGGCGACGAGCC
CGACTGGAGCGGCGACGGCACCGTGGAGATGGCGCCCAACGATGCCGAGTGGGGCGGCGACGCACCGCCGCGCACGCGCA
ACGATAGCGAGGGCGATGAGGCCGATGCCACCGAGCTGGCCCGCTCGCACGAATCACTCACTGCATTTCTGCACCGGCAG
GCCCTGGGATTGCGCTTGTCCGAGGTGGACTGTGCCGCCCTGCGCTTCCTGATCGAATCGCTGAACGACGACGGTTACCT
GGAAGAGTCGCTGGAGGAGCTGGCCATCACGCTCGCCGGCCCGGACGACCTGGAGCAGATCGAGGAGCTGGTGCACCGCT
TCACCGTGGCGCAGCGCCTGCTGCAGAGCCTGGAACCCGTGGGTGTGGGCGCCCGCGGCCTGGCCGAATGCCTGACCCTG
CAACTGCAGGCCATGGCCGCCGACGAAGACGGCCCCGCCGATGCCGCCACCGTGCAGACCGCGCTGCGCATCTGTCAGCA
GCCGCTGGAGATGCTGGCCAAGCGCGACGTGCGCCGCCTGACGGTGCTGTGCAGCGAGGGCGGTGCCAGCGGCGAAGAGC
GCACGCGCGCCGCCATGGCGCTGATTGCCCGGCTGGAGCCACGCCCGGGGCGCCGCTTTGCCGACGTGGAGCGCAACATC
ATCATTCCTGACGTGATCGTGCGCAAGGCGGGCCGCGAAGGCGCGCAAAGGGGCCGCGACACGCAGCAGAACTTCATCGT
GCAGCTCAACCCCGACGTGATGCCCCGCCTGCGCGTGCACGACATTTACGCAGGCGCCCTGCGCGGCCACAAGGGCGGCG
AGGGCCACCAGGGCATGCAGCAGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGGTTTGACACCATC
CTGCGCGTCTCGCGCGCCATCGTCGAGCGGCAAAAGAGCTTCTTCACCCACGGCGAGCTGGCCATGCGGCCGCTGGTGCT
GCGCGACATTGCCGACGAACTGGGCCTGCACGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCA
TTGGTACCTACGAGCTCAAGTATTTCTTTGGCTCGGGGCTGGGCACCGAAACCGGCGGCAATGCGTCGAGCACGGCGGTG
CGCGCGCTCATCAAGCAGTTTGTCGCCGCCGAAAATGCGGCCAAGCCGCTGTCCGACAGCCAGATCGCCGAGATGCTCAA
GGAGCAGGGCATCGAATGCGCCCGGCGCACCGTGGCCAAATACCGCGAAGCCCTCAAGATTGCGCCGGCCAACCTGCGCA
AGACGCTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(379-534)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-363)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3PRB9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.206

100

0.53

Multiple sequence alignment