Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BA891_RS13485 Genome accession   NZ_CP016347
Coordinates   2966244..2967260 (-) Length   338 a.a.
NCBI ID   WP_065302946.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16371     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2961244..2972260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA891_RS13460 (BA891_13460) aroK 2962116..2962634 (-) 519 WP_014233147.1 shikimate kinase AroK -
  BA891_RS13465 (BA891_13465) pilQ 2962823..2964562 (-) 1740 WP_065302943.1 type IV pilus secretin PilQ Machinery gene
  BA891_RS13470 (BA891_13470) pilP 2964594..2965109 (-) 516 WP_014233149.1 pilus assembly protein PilP Machinery gene
  BA891_RS13475 (BA891_13475) pilO 2965099..2965692 (-) 594 WP_065302944.1 type 4a pilus biogenesis protein PilO Machinery gene
  BA891_RS13480 (BA891_13480) pilN 2965685..2966260 (-) 576 WP_065302945.1 PilN domain-containing protein Machinery gene
  BA891_RS13485 (BA891_13485) pilM 2966244..2967260 (-) 1017 WP_065302946.1 type IV pilus assembly protein PilM Machinery gene
  BA891_RS13490 (BA891_13490) - 2967404..2969971 (+) 2568 WP_065302947.1 penicillin-binding protein 1A -
  BA891_RS13495 (BA891_13495) oxyR 2970035..2970943 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  BA891_RS13500 (BA891_13500) - 2971102..2971830 (+) 729 WP_065300291.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37641.83 Da        Isoelectric Point: 6.9713

>NTDB_id=187183 BA891_RS13485 WP_065302946.1 2966244..2967260(-) (pilM) [Vibrio natriegens strain CCUG 16371]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKNNYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIHCSVQESPLSGEEQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGRRALSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=187183 BA891_RS13485 WP_065302946.1 2966244..2967260(-) (pilM) [Vibrio natriegens strain CCUG 16371]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCGGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTAAGTCTGGACTTCGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCTGTTA
GCTACCAAGTTTTTGCGACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAACAACTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCATTGCTCTGTGCAGGAAAGCCCTCTGTCCGGAGAAGAACAAGCTAAAACGAGTCAATTTAAC
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCGATTAATGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTAGGAGGTCGCCGTGCTTTATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.852

100

0.749
  pilM Vibrio cholerae strain A1552

47.917

99.408

0.476

Multiple sequence alignment