Detailed information NTG0186791

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A0O31_RS06035
• Genome accession: NZ_CP016312
• Coordinates: 1152229..1153362 (-)
• Length: 377 a.a.
• NCBI ID: WP_071677089.1
• Uniprot ID: A0A1J0LTF0
• Organism: Thermus brockianus strain GE-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1147229..1158362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A0O31_RS06015 (A0O31_01237)	pilQ	1147795..1150071 (-)	2277	WP_071677086.1	secretin N-terminal domain-containing protein	Machinery gene
A0O31_RS06020 (A0O31_01238)	pilW	1150083..1151027 (-)	945	WP_071677087.1	competence protein	Machinery gene
A0O31_RS06025 (A0O31_01239)	pilO	1151024..1151623 (-)	600	WP_071677935.1	type 4a pilus biogenesis protein PilO	Machinery gene
A0O31_RS06030 (A0O31_01240)	pilN	1151613..1152236 (-)	624	WP_071677088.1	flagellar protein FliT	Machinery gene
A0O31_RS06035 (A0O31_01241)	pilM	1152229..1153362 (-)	1134	WP_071677089.1	type IV pilus assembly protein PilM	Machinery gene
A0O31_RS06040 (A0O31_01242)	-	1153555..1154559 (-)	1005	WP_071677090.1	homoisocitrate dehydrogenase	-
A0O31_RS06045 (A0O31_01243)	-	1154629..1155138 (-)	510	WP_071677091.1	DUF5317 domain-containing protein	-
A0O31_RS06050 (A0O31_01244)	-	1155174..1156547 (-)	1374	WP_420855815.1	HD-GYP domain-containing protein	-
A0O31_RS06065 (A0O31_01247)	-	1157459..1157824 (+)	366	WP_071677093.1	hypothetical protein	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41563.88 Da
• Isoelectric Point: 4.4814

>NTDB_id=186791 A0O31_RS06035 WP_071677089.1 1152229..1153362(-) (pilM) [Thermus brockianus strain GE-1]
MFSGLSKLFRPRVEALGLEVGASSLKLVELSGHPPTLRAYATRSIPPGTVVDGVVREPGTLAQEIRELLAEARTKKRYVV
SAVPNPALILRTLQVPKMPPKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVAEGEQMEVMVAAARQEAVASLIEAL
REAGLTPVVLDVKPFAGLYPLEEELNREPDRTVVAVEIGAESTSLVLTRGDRPLAVRLLTLSGKDFTEAIAKSFGLDFLT
AEDVKRTYGLATIPTEDEELLLDFDAERERYSPARIYDAIRPVLVELTQEIRRSLEFFRVQLGDIQPEVGYLYGGGSRLR
GLSTLLTDTLGVNFLVPDPWNGVQIDPKRFDLEKLREAGAELMVPVGLALRGVSPLD

Nucleotide

• Length: 1134 bp

>NTDB_id=186791 A0O31_RS06035 WP_071677089.1 1152229..1153362(-) (pilM) [Thermus brockianus strain GE-1]
GTGTTCTCGGGTTTGAGCAAGTTATTTAGACCTCGGGTGGAAGCGTTGGGCCTCGAGGTGGGGGCCTCCAGCCTCAAGCT
GGTGGAGCTTTCCGGCCACCCCCCAACGCTCAGGGCCTACGCCACCCGCTCCATTCCTCCTGGCACCGTGGTGGACGGCG
TGGTCCGGGAACCCGGTACTTTGGCCCAGGAAATCCGCGAGCTCCTGGCCGAAGCCCGGACTAAGAAGCGGTACGTGGTG
AGCGCCGTGCCCAATCCCGCCCTCATCCTGCGCACCCTCCAGGTGCCCAAGATGCCTCCCAAGGAGATGGAGGAAGCTGT
GCGATGGGAGGCGGAGCGCTACATCCCCTTCCCCATTGACGAGGTCGTCCTGGACTTCGCTCCCCTGGACCCTTTGGCGG
AGGTGGCCGAGGGGGAACAGATGGAGGTCATGGTGGCGGCGGCACGCCAGGAAGCCGTGGCCAGCCTCATAGAGGCCCTG
CGGGAAGCGGGGCTCACCCCCGTGGTCCTGGATGTCAAACCCTTCGCCGGGCTTTACCCTTTGGAGGAAGAGCTGAACCG
GGAGCCCGACCGCACCGTGGTGGCGGTGGAAATCGGGGCGGAAAGCACGAGCCTCGTCCTCACCCGGGGGGACCGCCCCT
TGGCGGTGCGCCTCCTCACCCTTTCGGGCAAGGACTTCACCGAGGCCATCGCCAAGAGCTTCGGCCTGGACTTCCTCACC
GCCGAGGACGTCAAGCGCACCTATGGCCTCGCCACCATCCCCACCGAGGACGAGGAACTCCTCCTGGACTTTGATGCCGA
AAGGGAGCGGTATAGCCCCGCCCGCATCTACGACGCCATCCGGCCCGTTCTGGTGGAGCTGACCCAGGAAATCCGCCGTA
GTTTGGAGTTCTTCCGGGTGCAACTCGGGGATATCCAGCCCGAGGTGGGTTACCTCTATGGCGGGGGAAGCCGGCTACGG
GGGCTTAGCACCTTGCTCACCGACACCCTAGGGGTCAACTTCCTGGTACCCGACCCTTGGAACGGGGTCCAGATAGACCC
CAAGCGCTTTGACCTGGAGAAGCTGCGGGAAGCAGGGGCTGAGCTCATGGTGCCCGTGGGCCTGGCCTTGAGGGGGGTGA
GTCCCCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-372)

3D structure

• AlphaFold DB: A0A1J0LTF0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	75.332	100	0.753