Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   BSK2_RS08520 Genome accession   NZ_CP020367
Coordinates   1637224..1639272 (+) Length   682 a.a.
NCBI ID   WP_029317792.1    Uniprot ID   -
Organism   Bacillus subtilis strain GQJK2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1632224..1644272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSK2_RS08490 (BSK2_08490) spoVM 1632860..1632940 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  BSK2_RS08495 (BSK2_08495) rpmB 1633013..1633201 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  BSK2_RS08500 (BSK2_08500) yloU 1633478..1633840 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  BSK2_RS08505 (BSK2_08505) fakA 1633856..1635517 (+) 1662 WP_038427652.1 DAK2 domain-containing protein -
  BSK2_RS08510 (BSK2_08510) sdaAB 1635656..1636318 (+) 663 WP_080529310.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  BSK2_RS08515 (BSK2_08515) sdaAA 1636344..1637246 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  BSK2_RS08520 (BSK2_08520) recG 1637224..1639272 (+) 2049 WP_029317792.1 ATP-dependent DNA helicase RecG Machinery gene
  BSK2_RS08525 (BSK2_08525) fapR 1639381..1639947 (+) 567 WP_003232044.1 transcription factor FapR -
  BSK2_RS08530 (BSK2_08530) plsX 1639961..1640962 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  BSK2_RS08535 (BSK2_08535) fabD 1640981..1641934 (+) 954 WP_080529311.1 ACP S-malonyltransferase -
  BSK2_RS08540 (BSK2_08540) fabG 1641927..1642667 (+) 741 WP_080529312.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  BSK2_RS08545 (BSK2_08545) acpP 1642751..1642984 (+) 234 WP_003154310.1 acyl carrier protein -
  BSK2_RS08550 (BSK2_08550) rncS 1643124..1643873 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78163.76 Da        Isoelectric Point: 7.4457

>NTDB_id=185345 BSK2_RS08520 WP_029317792.1 1637224..1639272(+) (recG) [Bacillus subtilis strain GQJK2]
MKQHQQTSIANIKGIGPETEKTLHELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=185345 BSK2_RS08520 WP_029317792.1 1637224..1639272(+) (recG) [Bacillus subtilis strain GQJK2]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTACACGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAATGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGCCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACAGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.853

100

0.999
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38