Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLUL8_RS10010 Genome accession   NZ_CP015908
Coordinates   1977254..1977805 (-) Length   183 a.a.
NCBI ID   WP_058221563.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain UL8     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1940223..1990911 1977254..1977805 within 0


Gene organization within MGE regions


Location: 1940223..1990911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUL8_RS09770 (LLUL8_1915) macP 1940223..1940501 (-) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  LLUL8_RS09775 (LLUL8_1916) - 1940494..1941078 (-) 585 WP_003130797.1 NUDIX hydrolase -
  LLUL8_RS09780 (LLUL8_1917) glmU 1941183..1942559 (-) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  LLUL8_RS09785 (LLUL8_1918) proC 1942787..1943575 (+) 789 WP_012898440.1 pyrroline-5-carboxylate reductase -
  LLUL8_RS09790 (LLUL8_1919) rpsO 1943654..1943923 (-) 270 WP_010906184.1 30S ribosomal protein S15 -
  LLUL8_RS09795 (LLUL8_1920) pknB 1944105..1945988 (-) 1884 WP_058221661.1 Stk1 family PASTA domain-containing Ser/Thr kinase -
  LLUL8_RS09800 (LLUL8_1921) - 1945988..1946764 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LLUL8_RS09805 (LLUL8_1922) rsmB 1946845..1948119 (-) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LLUL8_RS09820 (LLUL8_1923) - 1949249..1949977 (-) 729 WP_228763253.1 Abi family protein -
  LLUL8_RS09825 (LLUL8_1924) - 1950159..1950938 (-) 780 WP_082225246.1 peptidoglycan amidohydrolase family protein -
  LLUL8_RS09830 (LLUL8_1925) - 1950938..1951237 (-) 300 WP_082225247.1 phage holin -
  LLUL8_RS09835 (LLUL8_1926) - 1951250..1951600 (-) 351 WP_082225248.1 hypothetical protein -
  LLUL8_RS09840 (LLUL8_1927) - 1951613..1951849 (-) 237 WP_082225230.1 hypothetical protein -
  LLUL8_RS12770 (LLUL8_1928) - 1951861..1956213 (-) 4353 WP_145952585.1 hypothetical protein -
  LLUL8_RS09855 (LLUL8_1929) - 1956213..1957757 (-) 1545 WP_082225249.1 distal tail protein Dit -
  LLUL8_RS09860 (LLUL8_1930) - 1957760..1961527 (-) 3768 WP_237024039.1 tape measure protein -
  LLUL8_RS13315 - 1961734..1961841 (-) 108 WP_338141557.1 phage tail tube assembly chaperone -
  LLUL8_RS13320 - 1961910..1962134 (-) 225 Protein_1910 phage tail tube assembly chaperone -
  LLUL8_RS13185 (LLUL8_1932) - 1962201..1962428 (-) 228 WP_338141558.1 phage tail protein -
  LLUL8_RS13190 (LLUL8_1933) - 1962403..1962711 (-) 309 WP_237024041.1 phage tail protein -
  LLUL8_RS09875 (LLUL8_1934) - 1962753..1963148 (-) 396 WP_010905383.1 DUF806 family protein -
  LLUL8_RS09880 (LLUL8_1935) - 1963145..1963627 (-) 483 WP_021214764.1 HK97 gp10 family phage protein -
  LLUL8_RS09885 (LLUL8_1936) - 1963627..1963962 (-) 336 WP_010905381.1 phage head closure protein -
  LLUL8_RS09890 (LLUL8_1937) - 1963949..1964254 (-) 306 WP_010905380.1 head-tail connector protein -
  LLUL8_RS09895 (LLUL8_1938) - 1964265..1965578 (-) 1314 WP_058221420.1 phage major capsid protein -
  LLUL8_RS09900 (LLUL8_1939) - 1965568..1966146 (-) 579 WP_306472235.1 HK97 family phage prohead protease -
  LLUL8_RS09905 (LLUL8_1940) - 1966155..1967234 (-) 1080 WP_082225251.1 phage portal protein -
  LLUL8_RS13000 (LLUL8_1941) - 1967231..1967395 (-) 165 WP_058221580.1 hypothetical protein -
  LLUL8_RS09915 (LLUL8_1942) - 1967404..1969218 (-) 1815 WP_082225252.1 terminase large subunit -
  LLUL8_RS09920 (LLUL8_1943) - 1969218..1969613 (-) 396 WP_047687184.1 phage terminase small subunit P27 family -
  LLUL8_RS09925 (LLUL8_1944) - 1969770..1970273 (-) 504 WP_058221813.1 HNH endonuclease -
  LLUL8_RS12775 (LLUL8_1945) - 1970279..1970515 (-) 237 WP_058221812.1 hypothetical protein -
  LLUL8_RS09935 (LLUL8_1946) - 1970804..1971226 (-) 423 WP_032398555.1 RinA family protein -
  LLUL8_RS13005 (LLUL8_1947) - 1971751..1971906 (-) 156 WP_043991164.1 hypothetical protein -
  LLUL8_RS09945 (LLUL8_1948) - 1971903..1972088 (-) 186 WP_014570738.1 hypothetical protein -
  LLUL8_RS09950 (LLUL8_1949) - 1972096..1972299 (-) 204 WP_014570739.1 hypothetical protein -
  LLUL8_RS09955 - 1972422..1972598 (-) 177 WP_043991165.1 DUF1660 domain-containing protein -
  LLUL8_RS09960 (LLUL8_1950) - 1972595..1972813 (-) 219 WP_011834792.1 hypothetical protein -
  LLUL8_RS09965 (LLUL8_1951) - 1972860..1973105 (-) 246 WP_058221649.1 hypothetical protein -
  LLUL8_RS12850 (LLUL8_1952) - 1973127..1973276 (-) 150 WP_153927798.1 hypothetical protein -
  LLUL8_RS09970 (LLUL8_1953) - 1973273..1973947 (-) 675 WP_082225253.1 DUF1642 domain-containing protein -
  LLUL8_RS09975 (LLUL8_1954) - 1973940..1974146 (-) 207 WP_011676933.1 DUF1125 domain-containing protein -
  LLUL8_RS09980 (LLUL8_1955) - 1974160..1974684 (-) 525 WP_011834787.1 hypothetical protein -
  LLUL8_RS09985 - 1974793..1975032 (-) 240 WP_014570744.1 DUF1031 family protein -
  LLUL8_RS09990 (LLUL8_1956) - 1975025..1975219 (-) 195 WP_014570745.1 hypothetical protein -
  LLUL8_RS09995 (LLUL8_1957) - 1975222..1975611 (-) 390 WP_082225254.1 RusA family crossover junction endodeoxyribonuclease -
  LLUL8_RS10000 (LLUL8_1958) - 1975608..1976333 (-) 726 WP_058221565.1 hypothetical protein -
  LLUL8_RS10005 (LLUL8_1959) - 1976333..1977121 (-) 789 WP_058221564.1 phage replisome organiser protein -
  LLUL8_RS10010 (LLUL8_1960) ssb 1977254..1977805 (-) 552 WP_058221563.1 single-stranded DNA-binding protein Machinery gene
  LLUL8_RS10015 (LLUL8_1961) - 1977819..1978310 (-) 492 WP_050499054.1 ERF family protein -
  LLUL8_RS10020 (LLUL8_1962) - 1978319..1978708 (-) 390 WP_058221562.1 hypothetical protein -
  LLUL8_RS10025 (LLUL8_1963) - 1978710..1979042 (-) 333 WP_032398920.1 helix-turn-helix domain-containing protein -
  LLUL8_RS10030 (LLUL8_1964) - 1979039..1979275 (-) 237 WP_050499055.1 helix-turn-helix transcriptional regulator -
  LLUL8_RS10035 (LLUL8_1965) - 1979375..1979611 (-) 237 WP_032398921.1 DUF1408 domain-containing protein -
  LLUL8_RS10040 (LLUL8_1966) - 1979714..1980064 (+) 351 WP_032398922.1 hypothetical protein -
  LLUL8_RS10045 (LLUL8_1968) - 1980216..1980449 (-) 234 WP_011834776.1 hypothetical protein -
  LLUL8_RS10050 (LLUL8_1969) - 1980543..1980812 (-) 270 WP_011835841.1 hypothetical protein -
  LLUL8_RS10055 (LLUL8_1970) - 1980826..1981575 (-) 750 WP_058221874.1 phage antirepressor KilAC domain-containing protein -
  LLUL8_RS10060 (LLUL8_1971) - 1981587..1981817 (-) 231 WP_042749062.1 hypothetical protein -
  LLUL8_RS10065 (LLUL8_1972) - 1981922..1982188 (+) 267 WP_015966961.1 hypothetical protein -
  LLUL8_RS10070 (LLUL8_1973) - 1982182..1982376 (-) 195 WP_032398925.1 hypothetical protein -
  LLUL8_RS12855 (LLUL8_1974) - 1982416..1982625 (-) 210 WP_023164539.1 hypothetical protein -
  LLUL8_RS10080 (LLUL8_1975) - 1982779..1983156 (+) 378 WP_058221873.1 hypothetical protein -
  LLUL8_RS10085 (LLUL8_1976) - 1983149..1983415 (+) 267 WP_058221872.1 hypothetical protein -
  LLUL8_RS10090 (LLUL8_1978) - 1983584..1983781 (-) 198 WP_014735140.1 hypothetical protein -
  LLUL8_RS10095 (LLUL8_1979) - 1984011..1984547 (+) 537 WP_058221871.1 helix-turn-helix domain-containing protein -
  LLUL8_RS10100 (LLUL8_1980) - 1984553..1984987 (+) 435 WP_023164542.1 zinc peptidase -
  LLUL8_RS10105 (LLUL8_1981) - 1985001..1985336 (+) 336 WP_058221870.1 hypothetical protein -
  LLUL8_RS10110 (LLUL8_1982) - 1985404..1986585 (+) 1182 WP_058221869.1 site-specific integrase -
  LLUL8_RS10115 (LLUL8_1983) - 1986886..1987392 (-) 507 WP_003131479.1 Asp23/Gls24 family envelope stress response protein -
  LLUL8_RS10120 (LLUL8_1984) - 1987413..1987601 (-) 189 WP_012898445.1 DUF2273 domain-containing protein -
  LLUL8_RS10125 (LLUL8_1985) amaP 1987612..1988172 (-) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  LLUL8_RS10130 (LLUL8_1986) - 1988200..1988442 (-) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  LLUL8_RS10140 (LLUL8_1987) fmt 1988617..1989576 (-) 960 WP_010906187.1 methionyl-tRNA formyltransferase -
  LLUL8_RS10145 (LLUL8_1988) - 1989573..1990037 (-) 465 WP_003131474.1 VOC family protein -
  LLUL8_RS10150 (LLUL8_1989) - 1990003..1990524 (-) 522 WP_003131472.1 NUDIX hydrolase -
  LLUL8_RS10155 (LLUL8_1990) - 1990537..1990911 (-) 375 WP_014570768.1 nuclear transport factor 2 family protein -

Sequence


Protein


Download         Length: 183 a.a.        Molecular weight: 20998.76 Da        Isoelectric Point: 6.4657

>NTDB_id=183374 LLUL8_RS10010 WP_058221563.1 1977254..1977805(-) (ssb) [Lactococcus lactis subsp. lactis strain UL8]
MINNVVLVGRLTKDVELRYTPQNQATATFSLAVSRSFKNANGERETDFINCVIWRQQAENMANFTHKGSLIGITGRIQTR
NYENQQGQRVYVTEVVADSFQLLESRSQGQQQQQQGNYNQNQNNYQNQGQQNTVQQQHNQGNYQRQPQGNYQNQQQSAQQ
RAQQPDPNFGGAPMEINDEDLPF

Nucleotide


Download         Length: 552 bp        

>NTDB_id=183374 LLUL8_RS10010 WP_058221563.1 1977254..1977805(-) (ssb) [Lactococcus lactis subsp. lactis strain UL8]
ATGATAAATAACGTAGTTTTAGTCGGAAGACTAACTAAAGATGTAGAACTTAGATATACTCCACAAAATCAAGCTACAGC
AACTTTCTCGCTTGCAGTGAGTCGCTCTTTCAAAAATGCCAATGGAGAACGTGAAACAGATTTTATTAACTGTGTTATCT
GGCGACAACAAGCTGAAAATATGGCAAATTTCACACATAAAGGAAGCTTGATTGGTATCACTGGTAGAATCCAAACTCGA
AACTATGAAAATCAACAAGGACAACGTGTTTACGTTACTGAAGTTGTTGCAGATAGTTTCCAACTTCTTGAAAGTAGAAG
CCAAGGTCAACAACAGCAACAACAAGGAAATTACAATCAAAACCAAAATAATTACCAAAATCAGGGTCAACAAAATACTG
TTCAACAGCAACATAACCAAGGTAACTACCAAAGACAACCTCAAGGTAATTATCAAAATCAACAACAATCAGCTCAACAA
AGAGCCCAACAACCTGATCCAAACTTTGGAGGTGCTCCGATGGAAATCAACGATGAAGACCTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

55.851

100

0.574

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.514

100

0.541

  ssb Glaesserella parasuis strain SC1401

34.555

100

0.361


Multiple sequence alignment