Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   Eco28_RS00340 Genome accession   NZ_CP019944
Coordinates   77764..79845 (-) Length   693 a.a.
NCBI ID   WP_033815114.1    Uniprot ID   -
Organism   Escherichia coli strain DSM 103246     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 72764..84845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Eco28_RS00325 (Eco28_00065) yicH 72882..74591 (-) 1710 WP_062883739.1 AsmA family protein -
  Eco28_RS00330 (Eco28_00066) xanP 74712..76103 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  Eco28_RS00335 (Eco28_00067) gltS 76383..77588 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  Eco28_RS00340 (Eco28_00068) recG 77764..79845 (-) 2082 WP_033815114.1 ATP-dependent DNA helicase RecG Machinery gene
  Eco28_RS00345 (Eco28_00069) trmH 79851..80540 (-) 690 WP_001070174.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  Eco28_RS00350 (Eco28_00070) spoT 80547..82655 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  Eco28_RS00355 (Eco28_00071) rpoZ 82674..82949 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  Eco28_RS00360 (Eco28_00072) gmk 83004..83627 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76529.52 Da        Isoelectric Point: 7.3202

>NTDB_id=182302 Eco28_RS00340 WP_033815114.1 77764..79845(-) (recG) [Escherichia coli strain DSM 103246]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNITFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=182302 Eco28_RS00340 WP_033815114.1 77764..79845(-) (recG) [Escherichia coli strain DSM 103246]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCAGACTGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTACCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAATTGCAATATCACCTTCGGCGGCCGCCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACCATGCGCTTTTTCAACTTCAACGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTGCTGGCCTATGGAGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGTACGCCAGAGTTGCAGGAAACGCTCACGCCGGTTTATCCAACAACCGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACTGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTGCAACTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCATAACCTCAGCATGTTAGCCTTACGC
GCCGGAGCGCAACGTTTCCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAACTTCTTGCCGCCTTACCGTT
CAAGCCAACGGGCGCTCAGGCTCGCGTGGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCGCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGAACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGCTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGACACCTCGGTCATTGATGAACTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACTCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATG
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAACTACACCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCTGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)

DEAD/DEAH box helicase (DEAD)

(273-433)

RecG wedge domain (RecG_wedge)

(15-171)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.53

96.97

0.509
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.362

94.949

0.374

Multiple sequence alignment