Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   A7327_RS08445 Genome accession   NZ_CP015646
Coordinates   1733787..1735988 (-) Length   733 a.a.
NCBI ID   WP_049307743.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 08-02300     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1728787..1740988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7327_RS08430 (A7327_08425) lepA 1730291..1732114 (-) 1824 WP_000368345.1 translation elongation factor 4 -
  A7327_RS08435 (A7327_08430) rpsT 1732460..1732711 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  A7327_RS08440 (A7327_08435) holA 1732756..1733730 (-) 975 WP_001282561.1 DNA polymerase III subunit delta -
  A7327_RS08445 (A7327_08440) comEC 1733787..1735988 (-) 2202 WP_049307743.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A7327_RS08450 (A7327_08445) - 1735993..1736454 (-) 462 WP_000439693.1 ComE operon protein 2 -
  A7327_RS08455 (A7327_08450) comEA 1736546..1737232 (-) 687 WP_001788928.1 ComEA family DNA-binding protein Machinery gene
  A7327_RS08460 (A7327_08455) - 1737272..1737988 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  A7327_RS08465 (A7327_08460) rsfS 1737991..1738344 (-) 354 WP_001088020.1 ribosome silencing factor -
  A7327_RS08470 (A7327_08465) yqeK 1738345..1738929 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  A7327_RS08475 (A7327_08470) nadD 1738919..1739488 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  A7327_RS08480 (A7327_08475) yhbY 1739491..1739781 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  A7327_RS08485 (A7327_08480) aroE 1739785..1740591 (-) 807 WP_000666757.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 733 a.a.        Molecular weight: 84905.01 Da        Isoelectric Point: 10.0526

>NTDB_id=181762 A7327_RS08445 WP_049307743.1 1733787..1735988(-) (comEC) [Staphylococcus aureus strain 08-02300]
MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQ
VIQIQRQGSDTYKGRLSLKNEIYPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
HKQYIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYKPLKRLNLPLFVIKGIT
IIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQ
QLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFH
DWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTVWTIIILTLLITFPTNSHHKITMLNVGQGDSI
LYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKG
YSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLL
KKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
KVDSNSYGNASGL

Nucleotide


Download         Length: 2202 bp        

>NTDB_id=181762 A7327_RS08445 WP_049307743.1 1733787..1735988(-) (comEC) [Staphylococcus aureus strain 08-02300]
TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTCTCTACATTTCTTTTCATTTT
ACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGT
ATTTACATTATTCACAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAATGAAATATATCCTTTCTTTTT
AACAAATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGACA
ATAAGTTTGTAACTCTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
CATAAACAGTATATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATTATGGCATTGATTACTGGTGA
CGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGC
ATATAGCTGCAATTGTATTCTTAATTTACAAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
ATCATTGTATTAGCTTTATTTGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCTATAATAATGACAACTCTTGT
ACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAATC
CACTAGTTGTTTATGATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTTATCGTTGCCATTCCAAACTT
TCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTA
TTTTATTCTTTATTACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
GACTGGTTACTAGACCTATTCACAAGAATCAAGCAATCACATTTTTCTGTTCCCAAGTTTAATGATTGGATATTTATAAT
ATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCTAAACGTAAATATATATTGGTTACGGTTTGGACTATAATTA
TTCTGACATTATTAATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGATGATACTAAACAACCTAGTTA
TTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATC
CACACAATGACCATATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTTATGGATGTAAGACAAGTTAG
TAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGA
TTATTACTATGATTACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACAAGTAGTTCTAAAGAATTTAT
AGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTA
AACGATTGCAAAGGATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(183-441)

Metallo-beta-lactamase superfamily (Lactamase_B)

(474-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Staphylococcus aureus MW2

99.454

100

0.995
  comEC Staphylococcus aureus N315

99.045

100

0.99

Multiple sequence alignment