Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A7327_RS08205 Genome accession   NZ_CP015646
Coordinates   1689103..1689600 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 08-02300     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1684103..1694600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7327_RS08180 (A7327_08175) gcvPB 1684265..1685737 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  A7327_RS08185 (A7327_08180) gcvPA 1685730..1687076 (-) 1347 WP_000019692.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  A7327_RS08190 (A7327_08185) gcvT 1687096..1688187 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  A7327_RS08195 (A7327_08190) - 1688346..1688870 (-) 525 WP_001015120.1 shikimate kinase -
  A7327_RS08200 (A7327_08195) - 1688860..1689006 (-) 147 WP_001792109.1 hypothetical protein -
  A7327_RS08205 (A7327_08200) comGF 1689103..1689600 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  A7327_RS08210 (A7327_08205) comGE 1689518..1689817 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  A7327_RS08215 (A7327_08210) comGD 1689804..1690250 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  A7327_RS08220 (A7327_08215) comGC 1690228..1690539 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  A7327_RS08225 (A7327_08220) comGB 1690553..1691623 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  A7327_RS08230 (A7327_08225) comGA 1691595..1692569 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  A7327_RS08235 (A7327_08230) - 1692621..1693244 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  A7327_RS08240 (A7327_08235) - 1693241..1693570 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  A7327_RS08245 (A7327_08240) - 1693570..1694556 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=181756 A7327_RS08205 WP_001788897.1 1689103..1689600(-) (comGF) [Staphylococcus aureus strain 08-02300]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=181756 A7327_RS08205 WP_001788897.1 1689103..1689600(-) (comGF) [Staphylococcus aureus strain 08-02300]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment