Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A7327_RS08230 Genome accession   NZ_CP015646
Coordinates   1691595..1692569 (-) Length   324 a.a.
NCBI ID   WP_000697217.1    Uniprot ID   A0AAN2D5C1
Organism   Staphylococcus aureus strain 08-02300     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686595..1697569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7327_RS08190 (A7327_08185) gcvT 1687096..1688187 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  A7327_RS08195 (A7327_08190) - 1688346..1688870 (-) 525 WP_001015120.1 shikimate kinase -
  A7327_RS08200 (A7327_08195) - 1688860..1689006 (-) 147 WP_001792109.1 hypothetical protein -
  A7327_RS08205 (A7327_08200) comGF 1689103..1689600 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  A7327_RS08210 (A7327_08205) comGE 1689518..1689817 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  A7327_RS08215 (A7327_08210) comGD 1689804..1690250 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  A7327_RS08220 (A7327_08215) comGC 1690228..1690539 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  A7327_RS08225 (A7327_08220) comGB 1690553..1691623 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  A7327_RS08230 (A7327_08225) comGA 1691595..1692569 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  A7327_RS08235 (A7327_08230) - 1692621..1693244 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  A7327_RS08240 (A7327_08235) - 1693241..1693570 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  A7327_RS08245 (A7327_08240) - 1693570..1694556 (-) 987 WP_000161314.1 ROK family glucokinase -
  A7327_RS08250 (A7327_08245) - 1694553..1694756 (-) 204 WP_064626802.1 YqgQ family protein -
  A7327_RS08255 (A7327_08250) - 1694737..1696200 (-) 1464 WP_064626804.1 rhomboid family protein -
  A7327_RS08260 (A7327_08255) - 1696212..1696751 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  A7327_RS08265 (A7327_08260) rpmG 1696960..1697109 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36869.90 Da        Isoelectric Point: 8.8516

>NTDB_id=181761 A7327_RS08230 WP_000697217.1 1691595..1692569(-) (comGA) [Staphylococcus aureus strain 08-02300]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIAPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=181761 A7327_RS08230 WP_000697217.1 1691595..1692569(-) (comGA) [Staphylococcus aureus strain 08-02300]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGCACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTGGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAATTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment