Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BWL12_RS16545 Genome accession   NZ_CP019629
Coordinates   3171424..3172083 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain MGY     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3166424..3177083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWL12_RS16520 (BWL12_16780) ygiS 3167707..3169314 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  BWL12_RS16525 (BWL12_16785) mqsA 3169447..3169842 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  BWL12_RS16530 (BWL12_16790) mqsR 3169844..3170140 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  BWL12_RS16535 (BWL12_16795) ygiV 3170345..3170827 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  BWL12_RS16540 (BWL12_16800) ygiW 3170880..3171272 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BWL12_RS16545 (BWL12_16805) ciaR 3171424..3172083 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  BWL12_RS16550 (BWL12_16810) qseC 3172080..3173429 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  BWL12_RS16555 (BWL12_16815) ygiZ 3173475..3173807 (-) 333 WP_000917684.1 DUF2645 family protein -
  BWL12_RS16560 (BWL12_16820) mdaB 3174126..3174707 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BWL12_RS16565 (BWL12_16825) ygiN 3174738..3175052 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  BWL12_RS16570 (BWL12_16830) parE 3175100..3176992 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=181669 BWL12_RS16545 WP_001221495.1 3171424..3172083(+) (ciaR) [Escherichia coli strain MGY]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=181669 BWL12_RS16545 WP_001221495.1 3171424..3172083(+) (ciaR) [Escherichia coli strain MGY]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361