Detailed information NTG0181241

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: A3K93_RS06115
• Genome accession: NZ_CP015594
• Coordinates: 1290636..1291829 (-)
• Length: 397 a.a.
• NCBI ID: WP_067729881.1
• Uniprot ID: A0A172YNK6
• Organism: Acinetobacter sp. NCu2D-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1285636..1296829

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A3K93_RS06095 (A3K93_06095)	-	1287099..1287548 (+)	450	WP_067729873.1	MaoC family dehydratase	-
A3K93_RS06100 (A3K93_06100)	-	1287698..1288726 (+)	1029	WP_067729875.1	AraC family transcriptional regulator	-
A3K93_RS06105 (A3K93_06105)	gcvH	1288801..1289175 (+)	375	WP_067729877.1	glycine cleavage system protein GcvH	-
A3K93_RS06110 (A3K93_06110)	-	1289419..1290600 (+)	1182	WP_067729879.1	MFS transporter	-
A3K93_RS06115 (A3K93_06115)	pilU	1290636..1291829 (-)	1194	WP_067729881.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A3K93_RS06120 (A3K93_06120)	-	1292056..1293105 (-)	1050	WP_067729883.1	3-deoxy-7-phosphoheptulonate synthase	-
A3K93_RS06125 (A3K93_06125)	-	1293649..1294758 (-)	1110	WP_067729885.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
A3K93_RS06130 (A3K93_06130)	-	1294881..1296434 (+)	1554	WP_067729887.1	phospholipase D family protein	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44912.28 Da
• Isoelectric Point: 6.1577

>NTDB_id=181241 A3K93_RS06115 WP_067729881.1 1290636..1291829(-) (pilU) [Acinetobacter sp. NCu2D-2]
MFTADLLEEAKRFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQTLTAEKTKLFAYGLMNERQRQEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMSKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKGTRQNTASATAATEFTMYEEPKPEEDEDAEKESA

Nucleotide

• Length: 1194 bp

>NTDB_id=181241 A3K93_RS06115 WP_067729881.1 1290636..1291829(-) (pilU) [Acinetobacter sp. NCu2D-2]
ATGTTTACGGCAGATCTATTGGAAGAAGCGAAACGCTTTATGCACCATATGTTGACTAAAGTGGTGGAATACGGCGGCTC
CGATCTCTTTATTACTGCAGATTTCCCTCCAAGTATTAAGCATCAGGGATTGATGAAACCTTTCGGTCAGCAAACATTGA
CTGCTGAAAAAACCAAACTGTTTGCCTACGGCTTAATGAATGAGCGTCAACGTCAAGAATTTGAAACTGAACTCGAATGT
AACTTTGCCATTACCGTACCGGGTGTTTCGCGTTTCCGTGTCAATGTGTTTCAGCAACAGCTCAATGTCGGTATGGTCAT
TCGAACCATTACCGCTGAAATTCCAAACTTTCAAAAACTAAAACTTCCTGAGTCGCTCAAACATGTGATTATGGAAAAAC
GAGGTTTAGTTTTGGTCGTGGGTGGTACAGGTTCTGGTAAATCAACGTCACTTGCCGCCATGATTGACTATCGTAATGAA
AACTCTGCAGGGCATATCATTACCGTTGAAGACCCTGTTGAATACGTGCATAAACATAAAAAATCAATGATTACTCATCG
TGAAGTTGGCGTTGATTGCCACTCATGGCATAACGCGTTAAAAAATACTTTACGTCAAGCGCCCGATGTGATTTTGATTG
GTGAAATTCGTGATACCGAAACCATGGAGCATGCGATTGCCTTTGCTGAAACTGGGCATTTATGTTTAGGGACATTGCAT
TCTAATAATGCCAATCAAACTCTAGACCGAATTATTAACTTCTTCCCAGAAGAGCGCCGTAATCAGTTATTAATGGATTT
ATCATCCAATATGAAGGCGATTATTTCGCAGCGTTTGATTCGTACGGAAGATGGCAAAGGTCGTCGTGCTGCGATTGAAA
TTATGCTGAATACACCACTGATTTCAGATTTAATTTTAAAAGGCGAATTTCACGAACTTAAAGCAATTATGTCTAAGTCG
CGTGAATTGGGTATGCAAACTTTTGATCAGGCATTATTTGATCTGTATAACGAAGGCGCAATCTCGTATGAAGAAGCATT
ACGAAATGCTGATTCAACCAATGAATTACGTCTACAAATTAAATTAAAAGGGACACGCCAAAATACAGCAAGTGCAACCG
CTGCAACTGAATTTACCATGTATGAAGAGCCTAAACCTGAAGAAGATGAAGATGCTGAAAAGGAATCTGCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

3D structure

• AlphaFold DB: A0A172YNK6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.561	87.154	0.537
pilU	Acinetobacter baylyi ADP1	55.62	87.406	0.486
pilU	Vibrio cholerae strain A1552	52.113	89.421	0.466