Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BWZ24_RS05395 Genome accession   NZ_CP019355
Coordinates   708063..708722 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain XH988     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 703063..713722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWZ24_RS05370 (BWZ24_05875) parE 703154..705046 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  BWZ24_RS05375 (BWZ24_05880) ygiN 705094..705408 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  BWZ24_RS05380 (BWZ24_05885) mdaB 705439..706020 (-) 582 WP_203163758.1 NAD(P)H-dependent oxidoreductase -
  BWZ24_RS05385 (BWZ24_05890) ygiZ 706339..706671 (+) 333 WP_000912123.1 DUF2645 family protein -
  BWZ24_RS05390 (BWZ24_05895) qseC 706717..708066 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  BWZ24_RS05395 (BWZ24_05900) ciaR 708063..708722 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  BWZ24_RS05400 (BWZ24_05905) ygiW 708874..709266 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BWZ24_RS05405 (BWZ24_05910) ygiV 709319..709801 (+) 483 WP_000183494.1 AraC family transcriptional regulator -
  BWZ24_RS05410 (BWZ24_05920) parC 710347..712605 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  BWZ24_RS05415 (BWZ24_05930) plsC 712838..713575 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=180197 BWZ24_RS05395 WP_001221502.1 708063..708722(-) (ciaR) [Escherichia coli strain XH988]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=180197 BWZ24_RS05395 WP_001221502.1 708063..708722(-) (ciaR) [Escherichia coli strain XH988]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365