Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BE937_RS27420 Genome accession   NZ_CP019020
Coordinates   4827118..4827795 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain Ecol_244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4822118..4832795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE937_RS27405 (BE937_27980) chiA 4823476..4826178 (+) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -
  BE937_RS27410 (BE937_27985) bfd 4826347..4826541 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  BE937_RS27415 (BE937_27990) bfr 4826613..4827089 (+) 477 WP_000675504.1 bacterioferritin -
  BE937_RS27420 (BE937_27995) pilD 4827118..4827795 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  BE937_RS27425 (BE937_28000) gspM 4827795..4828256 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  BE937_RS27430 (BE937_28005) gspL 4828253..4829416 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  BE937_RS27435 (BE937_28010) gspK 4829431..4830414 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  BE937_RS27440 (BE937_28015) gspJ 4830407..4830994 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  BE937_RS27445 (BE937_28020) gspI 4830987..4831364 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  BE937_RS27450 (BE937_28025) gspH 4831361..4831870 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  BE937_RS27455 gspG 4831878..4832315 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=178254 BE937_RS27420 WP_000178170.1 4827118..4827795(-) (pilD) [Escherichia coli strain Ecol_244]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=178254 BE937_RS27420 WP_000178170.1 4827118..4827795(-) (pilD) [Escherichia coli strain Ecol_244]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382

Multiple sequence alignment