Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BTI679_RS20975 Genome accession   NZ_CP015257
Coordinates   4054894..4055328 (-) Length   144 a.a.
NCBI ID   WP_064474927.1    Uniprot ID   A0A1A9PZ83
Organism   Bacillus wiedmannii strain Bt18679     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4049894..4060328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI679_RS20955 (BTI679_42530) - 4050299..4050856 (+) 558 WP_064474931.1 PadR family transcriptional regulator -
  BTI679_RS20960 (BTI679_42540) - 4051047..4051478 (-) 432 WP_064474930.1 NUDIX hydrolase -
  BTI679_RS20965 (BTI679_42560) metE 4052142..4054430 (+) 2289 WP_064474929.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BTI679_RS20970 (BTI679_42570) comJ 4054468..4054857 (-) 390 WP_064474928.1 competence protein ComJ -
  BTI679_RS20975 (BTI679_42580) nucA/comI 4054894..4055328 (-) 435 WP_064474927.1 DNA-entry nuclease Machinery gene
  BTI679_RS20980 (BTI679_42590) - 4055566..4056315 (+) 750 WP_064474926.1 DUF3967 domain-containing protein -
  BTI679_RS20985 (BTI679_42600) kinB 4056354..4057628 (-) 1275 WP_000420381.1 sporulation sensor histidine kinase KinB -
  BTI679_RS20990 (BTI679_42610) - 4057938..4058138 (+) 201 WP_000929285.1 hypothetical protein -
  BTI679_RS20995 (BTI679_42620) - 4058262..4059425 (+) 1164 WP_000434595.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16070.43 Da        Isoelectric Point: 8.4331

>NTDB_id=178128 BTI679_RS20975 WP_064474927.1 4054894..4055328(-) (nucA/comI) [Bacillus wiedmannii strain Bt18679]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRSGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=178128 BTI679_RS20975 WP_064474927.1 4054894..4055328(-) (nucA/comI) [Bacillus wiedmannii strain Bt18679]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATACGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGATCGTAGTGGTGCTGCGGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGTGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCATGGGTAGGAAATAAGTTGGACAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A9PZ83

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment