Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BE939_RS05860 Genome accession   NZ_CP019008
Coordinates   916051..916710 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain Ecol_AZ159     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 911051..921710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE939_RS05830 (BE939_06010) ygiN 911951..912265 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  BE939_RS05835 (BE939_06015) mdaB 912296..912877 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BE939_RS27025 (BE939_06020) - 913128..913607 (+) 480 WP_061158005.1 Hcp family type VI secretion system effector -
  BE939_RS27030 (BE939_06025) - 913610..914320 (+) 711 WP_061158004.1 hypothetical protein -
  BE939_RS05850 (BE939_06030) ygiZ 914327..914659 (+) 333 WP_000914696.1 DUF2645 family protein -
  BE939_RS05855 (BE939_06035) qseC 914705..916054 (-) 1350 WP_000673367.1 quorum sensing histidine kinase QseC -
  BE939_RS05860 (BE939_06040) ciaR 916051..916710 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  BE939_RS05865 (BE939_06045) ygiW 916862..917254 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BE939_RS05870 (BE939_06050) ygiV 917307..917789 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  BE939_RS05875 (BE939_06055) ygiS 917898..919505 (+) 1608 WP_061158003.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=177934 BE939_RS05860 WP_001221493.1 916051..916710(-) (ciaR) [Escherichia coli strain Ecol_AZ159]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=177934 BE939_RS05860 WP_001221493.1 916051..916710(-) (ciaR) [Escherichia coli strain Ecol_AZ159]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCTCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365