Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   A4A60_RS20235 Genome accession   NZ_CP015222
Coordinates   3807229..3807348 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain HRBS-10TDI13     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3802229..3812348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4A60_RS20210 (A4A60_20220) albG 3802397..3803098 (+) 702 WP_069837830.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  A4A60_RS20215 (A4A60_20225) ywhL 3803104..3804480 (-) 1377 WP_069837831.1 YncE family protein -
  A4A60_RS20220 (A4A60_20230) ywhK 3804519..3805870 (-) 1352 Protein_3942 YncE family protein -
  A4A60_RS23875 (A4A60_20235) - 3805867..3806097 (-) 231 Protein_3943 hypothetical protein -
  A4A60_RS20230 (A4A60_20240) rapF 3806100..3807245 (+) 1146 WP_069837832.1 response regulator aspartate phosphatase RapF Regulator
  A4A60_RS20235 (A4A60_20245) phrF 3807229..3807348 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  A4A60_RS20240 (A4A60_20250) - 3807446..3807873 (+) 428 Protein_3946 YbaK/EbsC family protein -
  A4A60_RS20245 (A4A60_20255) speB 3807938..3808810 (-) 873 WP_003227545.1 agmatinase -
  A4A60_RS20250 (A4A60_20260) speE 3808871..3809701 (-) 831 WP_003227543.1 spermidine synthase -
  A4A60_RS20255 (A4A60_20265) pbpG 3809903..3811978 (+) 2076 WP_069837833.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=177691 A4A60_RS20235 WP_009968329.1 3807229..3807348(+) (phrF) [Bacillus subtilis strain HRBS-10TDI13]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=177691 A4A60_RS20235 WP_009968329.1 3807229..3807348(+) (phrF) [Bacillus subtilis strain HRBS-10TDI13]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487


Multiple sequence alignment