Detailed information NTG0177690

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: A4A60_RS20230
• Genome accession: NZ_CP015222
• Coordinates: 3806100..3807245 (+)
• Length: 381 a.a.
• NCBI ID: WP_069837832.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain HRBS-10TDI13
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3801100..3812245

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4A60_RS20205 (A4A60_20215)	albF	3801120..3802400 (+)	1281	WP_069837829.1	pitrilysin family protein	-
A4A60_RS20210 (A4A60_20220)	albG	3802397..3803098 (+)	702	WP_069837830.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
A4A60_RS20215 (A4A60_20225)	ywhL	3803104..3804480 (-)	1377	WP_069837831.1	YncE family protein	-
A4A60_RS20220 (A4A60_20230)	ywhK	3804519..3805870 (-)	1352	Protein_3942	YncE family protein	-
A4A60_RS23875 (A4A60_20235)	-	3805867..3806097 (-)	231	Protein_3943	hypothetical protein	-
A4A60_RS20230 (A4A60_20240)	rapF	3806100..3807245 (+)	1146	WP_069837832.1	response regulator aspartate phosphatase RapF	Regulator
A4A60_RS20235 (A4A60_20245)	phrF	3807229..3807348 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
A4A60_RS20240 (A4A60_20250)	-	3807446..3807873 (+)	428	Protein_3946	YbaK/EbsC family protein	-
A4A60_RS20245 (A4A60_20255)	speB	3807938..3808810 (-)	873	WP_003227545.1	agmatinase	-
A4A60_RS20250 (A4A60_20260)	speE	3808871..3809701 (-)	831	WP_003227543.1	spermidine synthase	-
A4A60_RS20255 (A4A60_20265)	pbpG	3809903..3811978 (+)	2076	WP_069837833.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45624.07 Da
• Isoelectric Point: 5.0624

>NTDB_id=177690 A4A60_RS20230 WP_069837832.1 3806100..3807245(+) (rapF) [Bacillus subtilis strain HRBS-10TDI13]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFTIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=177690 A4A60_RS20230 WP_069837832.1 3806100..3807245(+) (rapF) [Bacillus subtilis strain HRBS-10TDI13]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACAGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACATATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCACTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.688	100	0.987
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57